[Histonet] Tissue Orientation Markers

Paula Keene Pierce paula at excaliburpathology.com
Fri Mar 26 13:29:18 CDT 2021


You could use pieces of fixed/processed plant leaf embedded on edge.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

    On Friday, March 26, 2021, 01:23:39 PM CDT, Igor Deyneko via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Hello fellow Histonetters,

Has anyone ever worked with any type of a tissue orientation marker that is
not a dye, but rather some sort of a synthetic product that can be
processed and then sliced in a microtome?

Previously, we have used plain gut sutures and dyed histogel, which we
processed and made paraffin punch biopsies and then placed it as a tissue
marker along with tissues of interest and processed again. It's quite
cumbersome, so I'm trying to see if there are better options out there?

Thank you!

Igor Deyneko
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