[Histonet] IHC with zamboni's fixed tissues

[Tony Henwood (SCHN)]

Hi Colleen,

I was involved in an Immunohistochemical study of the nerves in the human ureter that used Zamboni's fixative and cryostat sections. This study was before the invention of HIER.

If you are using Zamboni-fixed, paraffin embedded tissue then you will probably need to do HIER before many of the localisation antibody incubations since the fixative contains 2% formaldehyde. The same might be the case with some antibodies when applied to cryostat sections.

Edyvane, K. A., Trussell, D. C., Jonavicius, J., Henwood, A., & Marshall, V. R. (1992). Presence and regional variation in peptide-containing nerves in the human ureter. Journal of the autonomic nervous system, 39(2), 127-137.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, 26 March 2021 9:42 AM
To: histonet-request <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] IHC with zamboni's fixed tissues

Hello Histonet,

Can you give me feedback on trying to do IHC on samples fixed in Zamboni's fixative? I have an idea but need confirmation .

Thank you in advance....

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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