[Histonet] Antibody diluent

Thu Mar 25 09:30:11 CDT 2021

I’m not completely sure but I think you can do either a mini validation or a comparison validation to show there’s no difference between the results.   

I think CAP has a requirement for what to do when reagents change.  

Paula

Sent from my iPhone

> On Mar 25, 2021, at 6:19 AM, Jeanine Ronkowski via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Scott:I’m pretty certain that you wouldn’t need to completely re-validate after changing diluents, but you may have to change the dilution factor slightly.  It’s my understanding that a change like this only requires staining a few known positive samples, verifying that the results you obtain are very similar if not identical to previous results.  While taking someone else’s suggestion to download that paper on diluents from Biocare’s site, I came across a paper on the subject of validation/verification that I found interesting.Jeanine
> 
> 
> Sent from Yahoo Mail for iPhone
> 
> 
> On Thursday, March 25, 2021, 8:30 AM, Lindrud, Scott <Scott.Lindrud at carrishealth.com> wrote:
> 
> What is everyone's take on what needs to be done from a verification/validation standpoint if you switch diluents for your concentrated antibodies?  The CAP summary of recommendations on the validation IHC antibodies doesn't specifically mention the changing of diluent as one of the variables.
> 
> If I change the diluent on my ER and Her2 concentrates, do I need to do a complete re-validation or just a quick verification using a few positive and negative cases?  I'm not really liking the idea of a complete re-validation!
> 
> Thanks for any info!
> 
> Scott
> 
> 
> Scott A. Lindrud, MLSCM(ASCP)CTCM  | Histopathology Technical Specalist/Cytotechnologist
> 
> P: 320-231-4520
> F: 320-231-4503
> scott.lindrud at carrishealth.com
> 
> Rice Memorial Hospital
> 301 Becker Ave SW
> Willmar, MN 56201
> 
> 
> 
> 
> -----Original Message-----
> From: Jeanine Ronkowski <jeanine.ronkowski at yahoo.com>
> Sent: Tuesday, March 23, 2021 2:21 PM
> To: Paula Keene Pierce <paula at excaliburpathology.com>; Maria Cruz <mcruz84pr at gmail.com>; Morken, Timothy <Timothy.Morken at ucsf.edu>
> Cc: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Antibody diluent
> 
> All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s really no such thing as a universal diluent like the ones offered by Leica and Agilent.  We get ours from Biocare, they offer five different diluents with different pHs and chemical makeups and we use three of these. I checked with my Biocare rep and iit seems that the one that’s most similar to Leicas is called Monet Blue.  I suggest you contact your Bocare rep and see if they can send you a sample of one or more them. Good  luck,Jeanine
> 
> 
> Sent from Yahoo Mail for iPhone
> 
> 
> On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> I just purchased antibody diluent from Electron Microscopy Sciences.
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com
> 
> A sharp knife is nothing without a sharp eye. - Klingon Proverb
> 
>     On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Maria, we went back to using Dako Diluent, which we had just switched away from to save money!
> 
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center
> 
> -----Original Message-----
> From: Maria Cruz via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Tuesday, March 23, 2021 10:55 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Antibody diluent
> 
> Like other folks in the histo community, I’ve recently experienced the inability to buy diluent from Leica.  Now wondering what others are doing to handle this problem.  TIA!
> Maria
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!-JVrniwUoxgIoDQOVTDi3LSafYCwpVCfe_opxTeRrS0Hkz2PmCjljTZnaAqQW-Mi7r3UYQ$
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents.
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet