[Histonet] Antibody diluent

[Lindrud, Scott]

What is everyone's take on what needs to be done from a verification/validation standpoint if you switch diluents for your concentrated antibodies?  The CAP summary of recommendations on the validation IHC antibodies doesn't specifically mention the changing of diluent as one of the variables.

If I change the diluent on my ER and Her2 concentrates, do I need to do a complete re-validation or just a quick verification using a few positive and negative cases?  I'm not really liking the idea of a complete re-validation!

Thanks for any info!

Scott


Scott A. Lindrud, MLSCM(ASCP)CTCM  | Histopathology Technical Specalist/Cytotechnologist

P: 320-231-4520
F: 320-231-4503
scott.lindrud at carrishealth.com

Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201




-----Original Message-----
From: Jeanine Ronkowski <jeanine.ronkowski at yahoo.com>
Sent: Tuesday, March 23, 2021 2:21 PM
To: Paula Keene Pierce <paula at excaliburpathology.com>; Maria Cruz <mcruz84pr at gmail.com>; Morken, Timothy <Timothy.Morken at ucsf.edu>
Cc: Histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Antibody diluent

All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s really no such thing as a universal diluent like the ones offered by Leica and Agilent.  We get ours from Biocare, they offer five different diluents with different pHs and chemical makeups and we use three of these. I checked with my Biocare rep and iit seems that the one that’s most similar to Leicas is called Monet Blue.  I suggest you contact your Bocare rep and see if they can send you a sample of one or more them. Good  luck,Jeanine


Sent from Yahoo Mail for iPhone


On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet <histonet at lists.utsouthwestern.edu> wrote:

I just purchased antibody diluent from Electron Microscopy Sciences.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb

    On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 Maria, we went back to using Dako Diluent, which we had just switched away from to save money!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

-----Original Message-----
From: Maria Cruz via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, March 23, 2021 10:55 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Antibody diluent

Like other folks in the histo community, I’ve recently experienced the inability to buy diluent from Leica.  Now wondering what others are doing to handle this problem.  TIA!
Maria
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