[Histonet] Bone decalcification

[Bryan Llewellyn]

Use surface decalcification:

Surface the block, then remove from the microtome. Do not use the 
microtome for another block, so as to avoid adjusting the position of 
the block face to the knife.

Place the block face down in 4% nitric acid for 2 hours or longer.

Rinse the block and cool down.

Place back into the microtome. Use a fresh blade to ensure sharpness. 
You should be able to get 3 or 4 sections at 5 microns before hitting 
the calcium again.

Bryan Llewellyn


Patricia Latham via Histonet wrote:
> To Histopeeps,
> Does anyone know if there is a method to decalcify bone once it is FFPE?
> Thank you,
> Pat L
> George Washington University
> Washington, DC
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