[Histonet] Unstained slides precut for IHC

Carrie Disbrow CDISBROW at msn.com
Sun Jun 13 21:04:34 CDT 2021


Hi! Current lab is cutting extra slides for IHC and putting in 60 degree oven for 45 mins to dry.  If IHC is ordered a precut control and the already baked slides are again put in the 60 degree oven for 45 mins. Sometimes the control is cut and added to the dry slide or a separate slide.  So, the questions  have been should the   slides be air dried first using a fan before baking and slides not baked until the pathologist places an order? Should the tissue and the control all have the same amount of time in the oven to ensure consistency? Also, is it better to rack slides standing or on edge for IHC? Additionally, when sending slides for IHC to other labs is it preferred to send dry slides or baked slides? Thanks for your input!
Carrie Disbrow, HTL

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