[Histonet] Re Protocol for DAPI staining on paraffin sections

[Hobbs, Carl]

Use DAPI/HOECHST at the same concentration you use for cells/FS
I use Hoechst H33258 Sigma 10mg/ml soln
I dilute by adding 0.5 microL to 100 microL buffer then add 1/100 to Alexa secondaries ( final 1/20K diln factor)
Some incubate in HOECHST or DAPI after secondary ab incubation, for IF
Sure, if only "staining" nuclei, incubate in 1/20K Hoechst in same buffer
This will vary for each lab
Or, buy mounting medium that contains DAPI

Good luck
Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813