[Histonet] Histonet Digest, Vol 212, Issue 7

[richard.wild at wanadoo.fr]

> Message: 1
> Date: Wed, 14 Jul 2021 22:03:24 +0000 (UTC)
> From: Cheryl <tkngflght at yahoo.com>
> To: "histonet at lists.utsouthwestern.edu"
> 	<histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] PathCentre (ThermoShandon) protocols - help?
> Message-ID: <7393119.276682.1626300204430 at mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
>
> Help?? Starting a refurbished PathCentre and was hoping someone could share a biopsy protocol for xylene processing?? Routine human biopsies with specials and some IHC and molecular down the road.
> Open to all -?
> Much appreciated!!
> Cheryl Kerry, HT(ASCP)

Hi Cherryl

it's a good machine (we have 3 of them)

Here under some "official" protocole

If unreadable, i'll send you pdf - just ask for it

Richard Wild

-----------------

SHANDON PATHCENTRE PROCESSING SCHEDULE
RAPID BIOPSY PROGRAMME TOTAL DURATION 1hr 14mins
STEP REAGENT CONC
%
TEMP
°C
VAC/PRES TIME
Hrs:Mins
DRAIN AGIT
1 Formalin 10 45 V 00:01 15 S
2 Formalin 70 45 V 00:01 15 S
3 Alcohol 95 45 V 00:01 15 S
4 Alcohol 100 45 V 00:01 15 S
5 Alcohol 100 45 V 00:01 15 S
6 Alcohol 100 45 V 00:01 15 S
7 Alcohol 100 45 V 00:01 15 S
8 Xylene 45 V 00:01 15 S
9 Xylene 45 V 00:01 15 S
10 Xylene 45 V 00:01 15 S
11 Wax 60 V 00:01 60 S
12 Wax 60 V 00:01 60 S
13 Wax 60 V 00:01 60 S
14 Wax 60 V 00:01 60 S

SHANDON PATHCENTRE PROCESSING SCHEDULE
RAPID PROCESSING TOTAL DURATION 0hr 56mins
STEP REAGENT CONC
%
TEMP
°C
VAC/PRES TIME
Hrs:Mins
DRAIN AGIT
1 Formalin 10 45 V 00:02 15 S
2 Formalin 70 45 V 00:02 15 S
3 Alcohol 95 45 V 00:02 15 S
4 Alcohol 00:00 S
5 Alcohol 00:00 S
6 Alcohol 100 45 V 00:02 15 S
7 Alcohol 100 45 V 00:02 15 S
8 Xylene 00:00 S
9 Xylene 45 V 00:02 15 S
10 Xylene 45 V 00:02 15 S
11 Wax 60 00:00 S
12 Wax 60 00:00 S
13 Wax 60 V 00:02 60 S
14 Wax 60 V 00:02 60 S

SHANDON PATHCENTRE PROCESSING SCHEDULE
LARGE SURGICALS TOTAL DURATION 22hrs 34mins
STEP REAGENT CONC
%
TEMP
°C
VAC/PRES TIME
Hrs:Mins
DRAIN AGIT
1 Buffered Formalin 10 A N 02:00 15 S
2 Alcohol Formalin 10 A N 02:00 15 S
3 90% IMS * 60 A V 01:30 15 S
4 IMS 80 A V 01:30 15 S
5 IMS A V 01:30 15 S
6 IMS A V 01:30 15 S
7 Xylene/IMS A V 01:30 15 S
8 Xylene A V 01:30 30 S
9 Xylene A V 01:30 60 S
10 Xylene A V 01:30 60 S
11 Wax 60 V 01:00 120 S
12 Wax 60 V 01:00 120 S
13 Wax 60 V 01:00 120 S
14 Wax 60 V 01:30 120 S