[Histonet] "cooked" biopsy

[Paula]

Thank you, everyone...
I looked at my reagents and saw the color pink in the xylene, which tells me that there is water or too much water in it so I changed it. 
We recycle xylene, so I need to get the recycler looked at now.
Thanks again,
Paula


-----Original Message-----
From: Erick Rodriguez [mailto:rodriguez.erick at icloud.com] 
Sent: Wednesday, July 07, 2021 11:24 AM
To: Paula
Subject: Re: [Histonet] "cooked" biopsy

Did you change the processor reagents before running your tissues? Cooked tissue usually means the tissue wasn’t dehydrated properly and the leftover water boiled and fried your tissue. I would double check the alcohols. 

> On Jul 7, 2021, at 11:00 AM, Paula via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Hello, good day,
> 
> 
> 
> Our pathologist is complaining about the tissues today that they are
> "cooked, burnt, crushed, shrunken" those are the adjectives she is using.  
> 
> 
> 
> Can you tell me the cause?  Usually, the work comes out beautiful but today
> they are not. Nothing has changed on our processing times.
> 
> 
> 
> What should I investigate?
> 
> 
> 
> Thank you in advance,
> 
> Paula
> 
> Bio-Path Medica Group
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet