[Histonet] Dipp Kwik Stain

[Tony Henwood (SCHN)]

There is a generally accepted scheme of staining which is expected of Romanowsky stained preparations, namely purple chromatin, blue leucocyte cytoplasm, purple-black basophil granules, red-pink eosinophil granules, purple neutrophil granules, purple platelet granules, and pink red-cells (1).

According to recommendations of the French Society of Clinical Cytology and of the French Association for Quality Assurance in Anatomic and Cytologic Pathology (2), red blood cells and polymorphonuclear leukocytes are the target cells for the quality evaluation of Romanowsky stains, and not the tumour cells. The tinctorial quality of Romanowsky stains is better and reliably judged on these target cells. Even if it is known that a ‘good MGG’ allows pink–orange (acidophilic) or buff-coloured RBCs to be obtained, many laboratories produce MGG slides showing blue or green erythrocytes, mainly because of a high (alkaline) pH of dilution/rinse solutions (3).

Horobin (3) has provided a useful table to trouble-shoot Romanowsky staining.

1.    Marshall PN, Bentley SA, Lewis SM. An evaluation of some commercial Romanowsky stains. Journal of clinical pathology. 1975 Aug 1;28(8):680-5.
2.    Piaton E, Fabre M, Goubin‐Versini I, Bretz‐Grenier MF, Courtade‐Saïdi M, Vincent S, Belleannée G, Thivolet F, Boutonnat J, Debaque H, Fleury‐Feith J. Guidelines for May‐Grünwald–Giemsa staining in haematology and non‐gynaecological cytopathology: recommendations of the French Society of Clinical Cytology (SFCC) and of the French Association for Quality Assurance in Anatomic and Cytologic Pathology (AFAQAP). Cytopathology. 2016 Oct;27(5):359-68.
3.	Horobin RW. How Romanowsky stains work and why they remain valuable—including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme. Biotechnic & Histochemistry. 2011 Feb 1;86(1):36-51.




Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

________________________________________
From: Estela Martinez via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Friday, 29 January 2021 02:53
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Dipp Kwik Stain

Hello All,

Do any of your pathologist use Dipp Kwik and if so, what kind of control do you use and do you document the control?  TIA!

Estela Martinez
Histology Supervisor
Medical Center Hospital
Odessa, TX 79761
432-640-2348
emartinez2 at echd.org
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