[Histonet] Tape Transfer Methods For Cryosectioned Brains

[Patpxs]

Good Morning Heather,

I have some  questions about how you cut frozen brains.   

What temperature are you cutting at?
How thick are your sections?
How are your samples frozen?  Flash freezing, slow freezing, iso-pentane in LN2? 

Your answers may provide clues to help you get better cryosections. 

Paula

Sent from my iPhone

> On Apr 15, 2021, at 5:39 AM, Heather Deziel via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Hello Histonet, 
> 
> I'm looking into working up a tape transfer method of collecting
> cryosections of brain while preserving infarct to be used in IHC.  I
> find that when I try and section heavily damaged regions of the brain
> the tissue tears and and I lose it.  Has anyone got any recommendations
> about the the Section-lab transfer tape (Kawamoto method), using the
> circuit plating tape recommended here
> (https://www.future-science.com/doi/full/10.2144/btn-2018-0021) or the
> cryojane system from Leica? 
> 
> Thank you, 
> 
> Heather
> 
> Heather Deziel, MSc.
> 
> Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A
> 4P3 
> 
>                  (a subsidiary of Neurodyn Life Sciences Inc.)
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