[Histonet] question on processing worms (11z at comcast.net)

[Hobbs, Carl]

Hi
I have done several yrs of work on Eisenia fetida worms with a PhD student.
I decided to do a std Pwax processing schedule.
The student dissected the appropriate area then placed in 10% Formalin in PBS pH7.4 for 24hrs on a rocker ( gentle agitation)
I then processed to Pwax using a std IMS/Xylene/wax on a Leica carousel ( 1hr in each station)
Worked fine.
Could do tinctorial and IHC no problem.
Sure, many primary Abs didn't work.......
We also did TUNEL on them Pwax sections....worked a treat.
I may have TUNEL images in the Histonet Image archive.

Good luck!
NB: Sure, maybe freezing is a better way forwards. Depends. 
All I know is that the PhD student passed with no problems and went on to a so far succesful career in Science Industry

Best wishes

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 


020 7848 6813