[Histonet] Vol 203, Issue 21

Fri Oct 30 14:21:12 CDT 2020

Hi Warda,

1. Both Roche and Leica have different instruments that are both equally
capable of breaking down.  It's really not feasible to determine which will
cost more in repairs on this premise alone.
2. Roche Ultra stains their probes with either a blue chromogen or you may
also use a Silver chromogen.

I hope this helps!
Eddie Martin

On Fri, Oct 30, 2020 at 3:30 AM warda hassan <redrose297 at gmail.com> wrote:

> Dear Eddie
>
> Thank you so much for your detailed explanation.
> Its really helpful, I just had two questions since you had worked on both
> the systems
>
> 1- Which one of them requires more maintenance and causes more breakdowns.
>
> 2- Does Roche performs FISH or is it SISH with probe IHC principle
>
> Thank you so much
> W Hassan
>
> On Fri, 30 Oct 2020 at 1:12 AM Eddie Martin <edmartin26 at gmail.com> wrote:
>
>> Hi Histonet and W.A. Hassan,
>>
>> Supposing you have a slide volume of 15-20k slides per year & currently
>> use a Dako instrument, but would like feedback on whether to get a:
>>                   * Bond Max, or
>>                   * Roche Ultra
>>
>> My response would depend more than what your yearly slide volume is.
>>                   * If...continually loading of slides while having
>> access to the reagent carousel is important to you, then I'd say Roche
>> Ultra has the advantage.
>>                   *If...having more control of your IHC protocols to
>> change your retrieval conditions, or even loading one time retrieval
>> conditions without saving it as a new protocol, then I'd say Leica Bond has
>> the advantage.
>>
>> It really depends on what your lab will be staining is what I'm getting
>> to.
>> Another factor to include is ease of use.  Roche Ultra is literally a lot
>> easier to use than Leica Bond, and Leica Bond is easier to use than the old
>> Dako's or Dako Flex or similarly Thermo or Biocare IHC instruments.
>>                    * If you're staining odd protocols and want an
>> instrument staining biomarker assays at variant conditions outside the
>> norm:
>>                      (ie: staining 50-100 micron frozen sections looking
>> for nerve bundles, or trying to perform FISH protocols on the instrument
>> without an installed FISH protocol on the instrument), then Leica is the
>> choice.
>>
>> quasi molecular staining:
>>                             1.   if your lab is interested in using m-RNA
>> oligo probes, choose Leica, as they have an easier setup to using different
>> vendors other than their own mRNA detection.
>>                             2.   if your physician staff prefers to see
>> probes with a matte background, only showing positive probe staining
>> (though using an older, yet very robust detection method), then Roche Ultra
>> has the advantage.
>>
>> If you're at a reference Lab and molecular performing testing that used
>> to be molecular based, but is now available via IHC, then Roche Ultra has a
>> huge advantage, particularly for the therapeutic and theranostic biomarkers
>> commercially available.
>>
>> Other factors to consider in your choice:
>>                     *Cost
>>                     * footprint of the instrument in your lab
>>                    * disposing of waste
>>                    * daily, weekly & monthly maintenance of the instrument
>>                    * cost of service.  Using a third party biomedical
>> group to repair or PM the instrument removes the IVD status of the
>> instrument, especially if you're staining any Class II's or higher, like
>> CD117, or ER, PR, Her2Neu...etc.
>>                                                 ***Its ok if you're just
>> using it for research if this doesn't apply.
>>
>> I hope this list helps you think what your team would like to use.  if
>> your pathologist and additional primary stakeholders would like to contact
>> me... you can.
>> I previously was a application specialist for IHC/probes with Leica
>> Microsystems up to 8 years ago.  However, I use a lot of Roche family of
>> instruments in my lab, as I'm a contracted lead for a well known
>> anatomic/clinical pathology laboratory.
>>
>> Best regards,
>> Eddie Martin, HT, HTL, QIHC
>>
>> On Thu, Oct 29, 2020 at 1:00 PM <
>> histonet-request at lists.utsouthwestern.edu> wrote:
>>
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>>> Today's Topics:
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>>>    1. Selection of IHC system (warda hassan)
>>>    2. Re: Selection of IHC system (Tina Van Meter)
>>>
>>>
>>>
>>> ---------- Forwarded message ----------
>>> From: warda hassan <redrose297 at gmail.com>
>>> To: "histonet at lists.utsouthwestern.edu" <
>>> histonet at lists.utsouthwestern.edu>
>>> Cc:
>>> Bcc:
>>> Date: Wed, 28 Oct 2020 22:48:14 +0400
>>> Subject: [Histonet] Selection of IHC system
>>> Dear Histonet
>>>
>>> Our lab is looking to purchase a new IHC system.
>>> Currently we are working with Dako.
>>> I would request your valuable feedback on :-
>>>
>>> 1- Bond max ( Leica)
>>> 2- Bench Mark Ultra (Roche Diagnostic)
>>>
>>> Workload is 15,000-20,000 slides/year.
>>>
>>> Many thanks in advance for your help.
>>>
>>> Kind regards
>>> W.A Hassan
>>>
>>>
>>>
>>>
>>> ---------- Forwarded message ----------
>>> From: Tina Van Meter <tina.vanmeter at gmail.com>
>>> To: warda hassan <redrose297 at gmail.com>
>>> Cc: Histonet at lists.utsouthwestern.edu
>>> Bcc:
>>> Date: Wed, 28 Oct 2020 23:08:07 -0400
>>> Subject: Re: [Histonet] Selection of IHC system
>>> Bond Max
>>>
>>> On Wed, Oct 28, 2020, 2:53 PM warda hassan via Histonet <
>>> histonet at lists.utsouthwestern.edu> wrote:
>>>
>>> > Dear Histonet
>>> >
>>> > Our lab is looking to purchase a new IHC system.
>>> > Currently we are working with Dako.
>>> > I would request your valuable feedback on :-
>>> >
>>> > 1- Bond max ( Leica)
>>> > 2- Bench Mark Ultra (Roche Diagnostic)
>>> >
>>> > Workload is 15,000-20,000 slides/year.
>>> >
>>> > Many thanks in advance for your help.
>>> >
>>> > Kind regards
>>> > W.A Hassan
>>> > _______________________________________________
>>> > Histonet mailing list
>>> > Histonet at lists.utsouthwestern.edu
>>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>> >
>>>
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>>
>>