[Histonet] Evaporation in frozen aliquots

[Hobbs, Carl]

Hi Kelly
If the tubes that hold your frozen ab aliquots do not have a complete seal....the water in them will lyophilise, over time.

I have your problem occasionally, for that reason ( I have a couple of thousand frozen primary abs)
Use snap-lid tubes ( I use 200microL PCR tubes)

Hope that explains.

Carl



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 


020 7848 6813