[Histonet] Evaporation in frozen aliquots

Hobbs, Carl carl.hobbs at kcl.ac.uk
Fri Oct 16 12:39:56 CDT 2020

Hi Kelly
If the tubes that hold your frozen ab aliquots do not have a complete seal....the water in them will lyophilise, over time.

I have your problem occasionally, for that reason ( I have a couple of thousand frozen primary abs)
Use snap-lid tubes ( I use 200microL PCR tubes)

Hope that explains.


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London

020 7848 6813

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