[Histonet] Alcian Blue staining

[Daniel Pesino]

Hi Charles - your control tissue may be autolyzed. Try using a well-processed control tissue. If that's not it, make sure that your control tissue is being completely deparaffinized and that it is being totally covered with the stains.

Good luck!

Daniel Pesino, HTL(ASCP)QIHC CM
Senior Histotechnologist
Trinity Health Of New England
Daniel.Pesino at TrinityHealthOfNE.org
W  860-714-4675

114 Woodland Street
Hartford, CT 06105
TrinityHealthOfNE.org | Facebook | Twitter | Instagram

-----Original Message-----
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Friday, October 9, 2020 10:14 AM
To: histonet at lists.utsouthwestern.edu
Subject: [External] [Histonet] Alcian Blue staining

Warning:  This email originated from the Internet!
DO NOT CLICK links if the sender is unknown, and NEVER provide your password.

We are having an issue where the goblet cells in our control tissue are not staining pink even though the patient tissue is staining beautifully on the same slide.

The stain is done manually.  What can be some causes for the issues in staining on the control section?
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Confidentiality Notice:
This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential.  Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.