[Histonet] Alcian Blue staining

Daniel Pesino Daniel.Pesino at TrinityHealthOfNE.org
Fri Oct 9 09:24:22 CDT 2020

Hi Charles - your control tissue may be autolyzed. Try using a well-processed control tissue. If that's not it, make sure that your control tissue is being completely deparaffinized and that it is being totally covered with the stains.

Good luck!

Daniel Pesino, HTL(ASCP)QIHC CM
Senior Histotechnologist
Trinity Health Of New England
Daniel.Pesino at TrinityHealthOfNE.org
W  860-714-4675

114 Woodland Street
Hartford, CT 06105
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-----Original Message-----
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Friday, October 9, 2020 10:14 AM
To: histonet at lists.utsouthwestern.edu
Subject: [External] [Histonet] Alcian Blue staining

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We are having an issue where the goblet cells in our control tissue are not staining pink even though the patient tissue is staining beautifully on the same slide.

The stain is done manually.  What can be some causes for the issues in staining on the control section?
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