[Histonet] Golgi staining problems

Alonso Martínez Canabal acanabal at ciencias.unam.mx
Thu Oct 8 13:02:43 CDT 2020

Good morning,
     Golgi-Cox method does not suit well with prefixed tissue. With short
fixation time in formaldehyde you have poorly stained neurons and many
astrocytes, with a prolonged fixation time you only see astrocytes.
     The silver nitrate method uses formaldehyde but I am not sure if it is
compatible with prefixation in formaldehyde.

El jue., 8 oct. 2020 a las 3:27, 蜗牛 via Histonet (<
histonet at lists.utsouthwestern.edu>) escribió:

> Can Golgi staining be performed with preperfused brain tissue? And after
> the completion of perfusion, the brain tissue has been cryopreserved for
> more than a year, can it still be used? What to do if available
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Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"

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