[Histonet] Histonet Digest, Vol 204, Issue 6

[Krista Ranalli]

Good morning All,
I have had my fair share of nails falling off. I have found sometimes
letting the slides air dry over night and staining then the next day helps.
Have you tried thus yet?
Respectfully
Krista

On Sat, Nov 14, 2020, 3:00 AM <histonet-request at lists.utsouthwestern.edu>
wrote:

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> Today's Topics:
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>    1. Toenail staining issues (Charles Riley)
>
>
>
> ---------- Forwarded message ----------
> From: Charles Riley <criley at dpspa.com>
> To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu
> >
> Cc:
> Bcc:
> Date: Fri, 13 Nov 2020 14:51:29 +0000
> Subject: [Histonet] Toenail staining issues
> >From time to time, we have issues with our sections of toenail falling
> off the slide during staining.
>
> Our H&E sections usually stain without issue but when we do our special
> stains (PASF and GMS) the tissue falls off routinely.
>
> We use charged slides and if a section falls off, we coat the recut slide
> with albumin to help get extra adherence. However sometimes even this does
> not help the tissue stick through the whole process (especially on GMS).
>
>
> Does anyone have any additional suggestion we could try to get the
> sections to adhere better throughout the process?
>
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