[Histonet] Toenail staining issues

[Bacon, Charles]

We use poly-L-lysine. Works for us.

P4707 Sigma-Aldrich
Poly-L-lysine solution
0.01%, sterile-filtered, BioReagent, suitable for cell cultur

Chuck Bacon, HTL(ASCP)CM 
Supervisor Histology
Baystate Medical Center
361 Whitney Ave., Holyoke, MA 01040
Telephone: 413-322-4786  Fax: 413-322-4790
Charles.Bacon at baystatehealth.org

-----Original Message-----
From: Charles Riley <criley at dpspa.com> 
Sent: Friday, November 13, 2020 9:51 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Toenail staining issues

>From time to time, we have issues with our sections of toenail falling off the slide during staining.

Our H&E sections usually stain without issue but when we do our special stains (PASF and GMS) the tissue falls off routinely.

We use charged slides and if a section falls off, we coat the recut slide with albumin to help get extra adherence. However sometimes even this does not help the tissue stick through the whole process (especially on GMS).


Does anyone have any additional suggestion we could try to get the sections to adhere better throughout the process?


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