[Histonet] DIF in paraffin section

[Morken, Timothy]

Manahil, we do IF on paraffin sections on occasion when kidney tissue is insufficient (and then only IgA, G, M and  K, L) or they want the markers on tissue that is only embedded in paraffin, such as kappa, Lambda for amyloid. We use at a higher concentration than for frozens (1:10 vs 1:100) and use pronase as an antigen retrieval agent. 

We use the Leica Bond stainer. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-----Original Message-----
From: MANAHIL EL BIREIR via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Friday, October 30, 2020 9:07 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] DIF in paraffin section

Hi Histonet:
Happy Halloween 🎃 
Would like to know if any one shift from DIF in fresh frozen section to embedded paraffin section!?
And which IHC auto machine are you using ?

Cheers,
Have a nice weekend 
Manahil 

Sent from my iPhone

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