[Histonet] [EXTERNAL] Brain and spinal cord

[Stedman, Nancy]

Hi – I use Excell Plus (alcohol based) for small brains (dog size and smaller).  I book them after 24 hours and then let them sit another 24 hours or more depending on the size, and changing the Excell.  It’s definitely not good as formalin, but my cases are veterinary necropsies and usually already have some autolysis already when they are submitted.  IHC seems to work just fine.  Have not tried ISH or nucleic acid extraction but the company that makes Excell Plus says it is suitable for these applications.  I have tried other alcohol based fixatives for small brains and none have been as good as Excell Plus.

-Nancy Stedman    


-----Original Message-----
From: Yahoo via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Friday, May 15, 2020 4:43 PM
To: histonet at lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] Brain and spinal cord

Hi All! 
I’m looking for some suggestions please on fixation for brain tissue and spinal cord submissions from necropsies. We are currently using 10% NBF and ask our pathologists to leave the samples overnight (but that doesn’t always happen!!). Does anyone use alcohol-based fixatives? And if so, how long? Does it affect IHC or any other staining? Do you still process with other routine biopsies (14 hour program)?
Thanks!

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