[Histonet] p53

[John Garratt]

http://cpqa.ca/assessments/Run%2095%20p53%20Summary%20(final).pdf

The above is an EQA report from CPQA /CIQC for p53 which gives specific protocols. In addition, if you go to the end of the report, there are the reporting guidelines for p53.
EQA reports are also available for other antibodies when you go to our web site.


John


www.cpqa.ca

‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Monday, May 4, 2020 7:40 AM, Jacque Sagasser via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Hello,
>
> Our lab has been using this down time to validate a few antibodies. So far we have validated Ki67, AE 1/3, CK20, and CDX-2. I am in the process of validating the P53 (DO-7) antibody, but am having trouble producing positive expression. I am using the same colon adenocarcinoma tissue I used to validate all of the antibodies that I previously mentioned. We are using the Ventana Benchmark XT along with the OptiView DAB. The package insert says to use colon adenocarcinoma for a control tissue, and the iView DAB. In the past, I have worked at a facility that ran it using the UltraView DAB so I don't think using the OptiView DAB is the issue. My Roche representative said he has heard of customers having to use a different control for the P53. Do you have any suggestions for controls or tweaking the protocol for this antibody given the parameters we are using? Any advice would be appreciated.
>
> Jacque R. Sagasser, HT (ASCP)cm
> Gandhi GI Pathology, LLC
> Kettering OH
>
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