[Histonet] Histonet Digest, Vol 198, Issue 1

[King,Michael A]

Make sure sodium azide (antimicrobial preservative) is not used in any of your IHC reagents--it inhibits peroxidase.
Mike

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Message: 3
Date: Fri, 1 May 2020 08:00:49 -0700
From: Kristy Castillo <k.castillo at azdermgroup.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC DAB
Message-ID:
        <CADFdJFAPWF_puvNe+ATKDwWoguN4GueLYq=nOeXa-uDDoN+ugw at mail.gmail.com>
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Hi Histonetters,

We are starting our IHC (fun times), we are having trouble with the DAB
lighting up.  Processed for 5 thru 10 minutes and still nothing.  Our
permanent red is working just fine.  Any ideas.

Thank you!

Kristy