[Histonet] Fw: [EXTERNAL] Pap slides

Tony Henwood (SCHN) tony.henwood at health.nsw.gov.au
Sat Mar 14 15:57:52 CDT 2020

We regularly leave smeared slides in 95% overnight and often over the weekend before staining.
There have not been any morphological issues noted.

Remember to seal the lid since alcohol will evaporate quite quickly. You will notice an increased eosinophilia in the dried upper area of the slide.

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

From: Perl, Alison via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Saturday, 14 March 2020 04:19
To: 'Charles Riley via Histonet'
Subject: Re: [Histonet] [EXTERNAL]  Pap slides

We routinely leave our ThinPrep Paps in 95 overnight and stain the next day. If I remember our Hologic training correctly, they said the slides can be left up to 5 days - best to check with an FAS before trying it, though

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
(914) 302-8424
aperl at caremount.com

-----Original Message-----
From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, March 13, 2020 12:52 PM
To: Histo List
Subject: [EXTERNAL] [Histonet] Pap slides

Can pap slides be left in 95% alcohol overnight to be stained the next
morning without affecting the quality of the slides? Or should they be
moved to xylene then run back prior to staining?


Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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