[Histonet] Anyone having difficulty with tissue adhesion?

[Hobbs, Carl]

I am very interested.
I do IHC on Pwax sections of mouse bone, human keloid tissue ( got a project aching to start which hopefully will result in a publication) and mouse/rat contused spinal cord.
All are quite difficult to keep on the slides as I have to, mostly, use HIER to get the best IHC- positive result.
The best slides I have used to date are Trajan's Series 3.
( superior to commercial Superfrost Plus, homemade Silanated or double-subbed slides)
However, they do not retain bone/cartilage...sure, keloid collagen is much better retained but there is still some loss
Sure, I get researchers to buy only them for the tissues stated above.
I will not be returning to work until 3rd August....maybe later.
It depends...….

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 
020 7848 6813


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 

020 7848 6813