[Histonet] stored in 80% isopropanol

[Betsy Molinari]

That is very interesting. Thank you for mentioning that!


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812 
Email: BMolinari at texasheart.org
texasheart.org <www.texasheart.org> | facebook <www.facebook.com/Texas.Heart.Institute> | twitter <twitter.com/Texas_Heart>
-----Original Message-----
From: Gilchrist, Eric P <Eric_Gilchrist at unc.edu> 
Sent: Wednesday, June 3, 2020 3:14 PM
To: Tracy Pheneger <Tracy.Pheneger at Colorado.EDU>; Betsy Molinari <BMolinari at texasheart.org>; Porter, Amy <portera at msu.edu>
Subject: RE: stored in 80% isopropanol

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If I am not mistaken, Thermo-Fisher's "Reagent Alcohols" are all Isopropanol based.  When we have providers who do not have a biopsy kit available and have a specimen I usually advise them to put the tissue in 70% isopropanol to fix and store and then overnight a biopsy kit to them.

Eric Gilchrist | Supervisor Oral & Maxillofacial Pathology University of North Carolina Adams School of Dentistry P 919.537.3153 | F 919.843.6508



-----Original Message-----
From: Tracy Pheneger via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, June 3, 2020 3:00 PM
To: Betsy Molinari <BMolinari at texasheart.org>; Porter, Amy <portera at msu.edu>; 'Histonet at lists.utsouthwestern.edu' <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] stored in 80% isopropanol

Isopropanol processing is routinely used with formalin fixation.
Isopropanol is miscible in paraffin, so you can skip the clearing agents. Just run it down to 100% Isopropanol as you would do on a Ethanol processing schedule, then straight into paraffin.
Check this out:

https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_14534624_&d=DwIGaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=Z8lCQROWymUZDTR2-MB-L6oGzMGD3l5tsvfWpQLIcGs&s=yJZyBp1_0VSLcTPQXOs29ban8nT7qsaQzMbA9StpsRA&e=

😊

Tracy

Tracy Pheneger, HT/HTL (ASCP), QIHC (ASCP), LATg (AALAS) Senior Professional Research Assistant BioServe Space Technologies University of Colorado c/o Kolicko AES Univ of Colorado Rm 224, 3775 Discovery Dr Boulder CO 80309

From: Betsy Molinari via Histonet<mailto:histonet at lists.utsouthwestern.edu>
Sent: Wednesday, June 3, 2020 12:50 PM
To: Porter, Amy<mailto:portera at msu.edu>; 'Histonet at lists.utsouthwestern.edu'<mailto:histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] stored in 80% isopropanol

I am also in research. I have very little to go on, but I presume it is not going to yield good results.


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
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From: Porter, Amy <portera at msu.edu>
Sent: Wednesday, June 3, 2020 1:47 PM
To: Betsy Molinari <BMolinari at texasheart.org>
Subject: Re: stored in 80% isopropanol


*** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe.

________________________________

Rubbing alcohol - potentially really dehydrated already depending on how long???  I am research so no recommendation for clinical.....

________________________________
From: Betsy Molinari via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Sent: Wednesday, June 3, 2020 2:39 PM
To: 'Histonet at lists.utsouthwestern.edu' <Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu>>
Subject: [Histonet] stored in 80% isopropanol

Hi,
If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol  can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol?
Thanks,
Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston, TX 77030

832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org<mailto:BMolinari at texasheart.org>
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