[Histonet] Histonet Digest, Vol 200, Issue 19

Madeleine Huey madeleinehuey at gmail.com
Thu Jul 30 13:06:25 CDT 2020 

Original Message-----
From: Ken M via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, July 28, 2020 11:43 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Apple Green Birefringence in Amyliod slides

Hi Ken,
May I give you my personal experience on Congo Red?
1) Always cut thick tissue (ie,10um thickness, but 8 um will work)
2) Always fresh cut tissue. You can pre-cut control slides, but store them
in the *dark* @ *4C (ie. fridge, never RT)*.
Amyloid will fade if pre-cut and stored at room temperature with light.  We
always stored our stained control slide as reference along with our pre-cut
unstained slides in the fridge, but "apple green birefringence" will also
fade with time (unstable).


*Madeleine Huey, **B.S., HTL & QIHC (ASCP)*

Supervisor, Pathology/Histology & IPOX (MV & LG)

El Camino Hospital

2500 Grant Road (Rm GC-33)

Mountain View, CA 94040

Phone:  650-940-7038

Fax:        650-988-8387

Email:  madeleine_h at elcaminohospital.org


Hi everyone.  I was wondering if anyone out there has any experience with
diagnosing Amyloid tissue using Congo Red stained Kidney using polarized
lenses.  Is it common to use polarized light to detect Amyloid deposits?
Does the absence of the "apple green birefringence" indicate a problem with
the control tissue or the control slides?  Should this green bifringence
always appear to confirm the diagnosis?  I know that the tissue should be
cut thicker than normal (we usually cut at 5), but in the future maybe we
will cut at 7 or 8?

On Thu, Jul 30, 2020 at 10:04 AM <histonet-request at lists.utsouthwestern.edu>
wrote:

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> Today's Topics:
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>    1. Re: Apple Green Birefringence in Amyliod slides (John Kiernan)
>    2. Sakura Tissue-Tek versus Tanner embedding station Comparison
>       (Rob Rankin)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 29 Jul 2020 18:39:31 +0000
> From: John Kiernan <jkiernan at uwo.ca>
> To: Ken M <kdean70 at hotmail.com>, "Morken, Timothy"
>         <Timothy.Morken at ucsf.edu>
> Cc: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides
> Message-ID:
>         <
> YTOPR0101MB161161FC59817A6B556CCDEFA5700 at YTOPR0101MB1611.CANPRD01.PROD.OUTLOOK.COM
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> For another source of polarizing filters, go to a 3D movie, take home the
> glasses they provide, and poke out the lenses. They work very nicely as
> polarizer and analyzer with an ordinary microscope.
> John Kiernan
> Anatomy & Cell Biology
> University of Western Ontario
> London, Canada
> = = =
> ________________________________
> From: Morken, Timothy via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: 28 July 2020 13:57
> To: Ken M <kdean70 at hotmail.com>
> Cc: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides
>
> Ken, Yes, polarized light and apple green birefringence is diagnostic for
> amyloid with congo red and is the best practice. If you have a problem with
> known control slides  there are two possibilities: 1) make up fresh
> solution. The pH has to be right. Or 2) try other control slides. Maybe you
> cut through the amyloid area.
>
> Because we have hundreds of microscopes in our department most just use
> polarized film as the polarizer (put over the light source) and another put
> over the top of the slide as the analyzer. Turn one of the polarizing
> slides and you will see the birefringence appear.
>
> Source:
> "Polarizing film, 2"" x 2"" , PK/10 (BEST For use as a microscope
> polarizer)"   Cat# S07372     Thermo Fisher Sci Health        $36.75
> PK/10   "2" x 2"
>
> These are polarized film mounted in 2" film holders (like the old
> Kodachrome slides).
>
> Cheap and effective. (and avoids consternation from people losing
> expensive microscope polarizers)
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
> -----Original Message-----
> From: Ken M via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Tuesday, July 28, 2020 11:43 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Apple Green Birefringence in Amyliod slides
>
> Hi everyone.  I was wondering if anyone out there has any experience with
> diagnosing Amyloid tissue using Congo Red stained Kidney using polarized
> lenses.  Is it common to use polarized light to detect Amyloid deposits?
> Does the absence of the "apple green birefringence" indicate a problem with
> the control tissue or the control slides?  Should this green bifringence
> always appear to confirm the diagnosis?  I know that the tissue should be
> cut thicker than normal (we usually cut at 5), but in the future maybe we
> will cut at 7 or 8?
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> ------------------------------
>
> Message: 2
> Date: Thu, 30 Jul 2020 10:26:57 -0400
> From: Rob Rankin <robrankin at rankinbiomed.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Sakura Tissue-Tek versus Tanner embedding station
>         Comparison
> Message-ID:
>         <CAOEuZ=w1MRj4biRoW9a2muA9oDvWnw4T=
> Wmhh-Kh51DVHSt8Cg at mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Is anyone using a Tanner embedding station? If so, what has your experience
> been like? I'm trying to compare between a Sakura Tissue-Tek and Tanner
> (the Tanner is much cheaper).
>
> Respectfully,
> *Rob Rankin, MSM, SM(ASCP)*
> Founder & CEO
> Office: 248 625-4104 ext 0012
> www.rankinbiomed.com
>
> <https://www.rankinbiomed.com/product-categories/>
>
> 14515 Mackey Rd.
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>
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> afraid, neither be thou dismayed: for the LORD thy God is with thee
> whithersoever thou goest. *
>
> *Joshua 1:9*
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> End of Histonet Digest, Vol 200, Issue 19
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