[Histonet] Histonet Digest, Vol 200, Issue 9

Ken Urban kenu2 at comcast.net
Fri Jul 10 16:56:14 CDT 2020 

Charles Culling is the name/person you’re trying to remember.

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> On Jul 10, 2020, at 12:00 PM, histonet-request at lists.utsouthwestern.edu wrote:
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> Today's Topics:
> 
>   1. Re: Crystals forming in Hematoxylin (Hannen, Valerie)
>   2. Re: Crystals forming in Hematoxylin (Paula Keene Pierce)
>   3. Histology text book  "bibles" (Hobbs, Carl)
>   4. Re: Histology text book  "bibles" (Muhammad Azam)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Thu, 9 Jul 2020 17:09:28 +0000
> From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
> To: 'Terri Braud' <tbraud at holyredeemer.com>
> Cc: "Histonet at lists.utsouthwestern.edu"
>    <Histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Crystals forming in Hematoxylin
> Message-ID:
>    <d0a38ccca1e44aab95e176bc3b6d5ae3 at PMSVRLEX02.parrishmed.local>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi Terri,
> 
> The crystals form during cold winter months, usually during shipping or can form if the Hematoxylin 7211 is stored in a cold environment.  They also form due to the Mordant being put into solution at it's saturation point.
> The crystals have no adverse effect on the stain itself.  If your bottle has a few crystals, just filter it and it should remain crystal free.  If there are a lot, you can gently warm it, using a "hot" plate and constant stirring until the crystals go back into solution, then filter it and is should be good to go.   I only learned this when I "googled" about it myself.  I hope this helps!!
> 
> Regards,
> 
> Valerie
> 
> 
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.hannen at parrishmed.com
> www.parrishmed.com
> 
> 
> 
> -----Original Message-----
> From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
> Sent: Thursday, July 09, 2020 12:39 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [EXTERNAL Sender] [Histonet] Crystals forming in Hematoxylin
> 
> 
> This message came from an external source. Please do not click links or open attachments if unexpected or unusual.
> 
> Begin Original Message:
> 
> ----------------------------------------------------------------------
> Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?  We use Richard-Allen 7211.  We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom.  Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful.  It had a few occlusions of purple streaks, but otherwise was clear.  Doesn't seem to affect the stain, so I was just curious.
> Inquisitively yours, Terri
> 
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
> 
> _______________________________________________
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> Histonet at lists.utsouthwestern.edu
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> 
> 
> ------------------------------
> 
> Message: 2
> Date: Thu, 9 Jul 2020 17:21:29 +0000 (UTC)
> From: Paula Keene Pierce <paula at excaliburpathology.com>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>,    Terri Braud
>    <tbraud at holyredeemer.com>
> Subject: Re: [Histonet] Crystals forming in Hematoxylin
> Message-ID: <114995151.2633909.1594315289274 at mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
> 
> That is the aluminum salt precipitating out.
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com
> 
> A sharp knife is nothing without a sharp eye. - Klingon Proverb 
> 
>    On Thursday, July 9, 2020, 11:47:53 AM CDT, Terri Braud via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
> 
> Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?? We use Richard-Allen 7211.? We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom.? Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful.? It had a few occlusions of purple streaks, but otherwise was clear.? Doesn't seem to affect the stain, so I was just curious.
> Inquisitively yours, Terri
> 
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Thu, 9 Jul 2020 18:28:54 +0000
> From: "Hobbs, Carl" <carl.hobbs at kcl.ac.uk>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Histology text book  "bibles"
> Message-ID:
>    <AM6PR03MB3944FD589EE29D9A63891607C4640 at AM6PR03MB3944.eurprd03.prod.outlook.com>
>    
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Good to read of others' xp
> I remember the Armed Forces book...good in parts, unlike the Curate's egg!
> Disbrey and Rack...wonderfully refreshing.
> Exceedingly well-informed person was Brenda!
> I reckon she swung the panel in my favour for a Histology Manager job.....YEARS ago!
> Imho the forerunner of the best: Kiernan 
> Sure, neck and neck with Bancroft and Stevens....both game-changers
> Pearce...wonderfully instructive yet....dense?
> What about the 70's "bible"...I forget the English author......dash it!
> His surname begins with a C?
> Carleton!
> The book was THE text at that time ( in UK)...however, I found it rather didactic....no depth
> I could be wrong, of course!
> I did learn a lot from it...then found it lacing in depth...thirst for Histo teck knowledge, chuckle
> It gave me a seminal Taster for more, however.
> Met him at many London Histology discussion meetings...at one of which he dismisssed me for using DPX mountant
> ( cheaper for my lab/quickersetting...close RI , I recall?)
> Harrumph, he said....use natural resin: Canada balsam!
> Much closer RI....hmm
> What does Prof. Kiernan think re DPX v Canada balsam??
> Luverly smell..tho (  C.balsam)
> Does anyone use CBalsam still?
> Also....
> sure, Harry Cook has a special place in my Histology career...such a humble yet...knowledgeable person.
> He opened up my mind to things Histological...his depth and breadth of things Histological was...humbling and mesmeric.
> His Carbohydrate booklet was so good for me, when I needed it!
> Curious-illy
> Always
> 
> Carl
> 
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge?
> Kings College London
> London
> SE1 1UL
> ?
> 
> 020 7848 6813
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Thu, 9 Jul 2020 17:29:58 -0400
> From: Muhammad Azam <ajju33 at gmail.com>
> To: "Hobbs, Carl" <carl.hobbs at kcl.ac.uk>
> Cc: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Histology text book  "bibles"
> Message-ID: <9992E63F-8B3C-4E50-A2C4-43024BCA3AB9 at gmail.com>
> Content-Type: text/plain; charset=utf-8
> 
> These last few posts r so wonderful and revealing. Where we came from and all the glorious history. Very nostalgic
> 
> Dr Azam
> 
> 
> Sent from my iPhone
> 
>> On Jul 9, 2020, at 2:44 PM, Hobbs, Carl via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>> 
>> ?Good to read of others' xp
>> I remember the Armed Forces book...good in parts, unlike the Curate's egg!
>> Disbrey and Rack...wonderfully refreshing.
>> Exceedingly well-informed person was Brenda!
>> I reckon she swung the panel in my favour for a Histology Manager job.....YEARS ago!
>> Imho the forerunner of the best: Kiernan 
>> Sure, neck and neck with Bancroft and Stevens....both game-changers
>> Pearce...wonderfully instructive yet....dense?
>> What about the 70's "bible"...I forget the English author......dash it!
>> His surname begins with a C?
>> Carleton!
>> The book was THE text at that time ( in UK)...however, I found it rather didactic....no depth
>> I could be wrong, of course!
>> I did learn a lot from it...then found it lacing in depth...thirst for Histo teck knowledge, chuckle
>> It gave me a seminal Taster for more, however.
>> Met him at many London Histology discussion meetings...at one of which he dismisssed me for using DPX mountant
>> ( cheaper for my lab/quickersetting...close RI , I recall?)
>> Harrumph, he said....use natural resin: Canada balsam!
>> Much closer RI....hmm
>> What does Prof. Kiernan think re DPX v Canada balsam??
>> Luverly smell..tho (  C.balsam)
>> Does anyone use CBalsam still?
>> Also....
>> sure, Harry Cook has a special place in my Histology career...such a humble yet...knowledgeable person.
>> He opened up my mind to things Histological...his depth and breadth of things Histological was...humbling and mesmeric.
>> His Carbohydrate booklet was so good for me, when I needed it!
>> Curious-illy
>> Always
>> 
>> Carl
>> 
>> Carl Hobbs FIBMS
>> Histology and Imaging Manager
>> Wolfson CARD
>> Guys Campus, London Bridge 
>> Kings College London
>> London
>> SE1 1UL
>> 
>> 
>> 020 7848 6813
>> _______________________________________________
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
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> End of Histonet Digest, Vol 200, Issue 9
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