[Histonet] Fixed frozen non-paraffin mouse brain

[Tina Van Meter]

Hi Ed,
I have been using the 30% sucrose technique to cryoprotect animal tissue
for over 40 years without any problem. Did the tissue sink to the bottom of
the specimens jar? After sinking, I blot the excess sucrose from the tissue
on a paper towel before transfering to OCT. What is your procedure to
freeze the tissue?

Thank you,
Tina Van Meter
Scripps Research
Histology Core Manager
Jupiter, FL

On Sat, Jul 4, 2020, 9:26 PM Roy, Edward J via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> As a research lab, we sometimes would like to use paraformaldehyde-fixed
> but non-paraffin embedded tissues; paraffin embedding alters antigens and
> necessitates antigen retrieval, but simple fixation does not. We have done
> the traditional 30% sucrose before OCT and freezing, with cryostat
> sectioning, but results are inconsistent, sometimes producing Swiss-cheese
> brains. Does anybody have an alternative to 30% sucrose that is more
> reliable?  I didn’t see anything in the Archives after a search for “30%
> sucrose”.
> Thanks very much,
> Ed Roy
>
>
> Edward J. Roy, PhD
> Professor Emeritus
> Department of Molecular and Integrative Physiology
> University of Illinois at Urbana-Champaign
> Urbana, IL 61801
> 217 333-3375
>
>
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