[Histonet] Tissue processor errors, failures and what to do

[E. Wayne Johnson]

Automation is a wonderful thing but it is only a replacement for what people used to do by hand.
We have incubators that can be set to 60C and we have a Rube Goldberg-ized microwave oven with
a thermal controller and relays (and the not-to-be-forgotten flyback diode) and
a K-type thermal probe covered with aluminum foil (dont try this at home, kids)
so we are ready for any incident or mishap with our cantankerous VIP5.

There is no such thing as a convenient time for it to quit but lots of times the trouble
is that it was time to change the solutions and nobody did it even though
anybody could have done it.

If there is nothing we can do to get the VIP5 going again pronto
we finish the processing by hand.
there are repair manuals for some of those machines available online
or you can call the repairman.  If you are out in Bufooee somewhere the repair
manual and the secret repair modes on the machine could be the difference between joy
and the slough of despond.

E. Wayne Johnson DVM
Enable AgTech
Beijing

Patpxs via Histonet wrote:
> Hi Garrey,
>
> The answer is “it depends”.   What you do when a processor fails depends on the failure point.  If the tissue is still in dehydrant it gets treated differently than if it fails in the intermediate solvent.
>
> Paula
>
> Sent from my iPhone
>
>> On Jul 4, 2020, at 10:08 AM, Garrey Faller via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>>
>> Happy 4th to all.
>> Does anyone have a procedure on what to do when a tissue processor fails or alarms.  I want to learn more about the science behind tissue processing so I know what to do when the machine fails. This happened to a friend recently and I want to prevent my tissues/biopsies from being ruined.
>> Thanks.
>> Garrey
>>
>> Sent from my iPhone
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