[Histonet] Need a procedure

Patsy Ruegg pruegghm at hotmail.com
Tue Jan 28 15:31:05 CST 2020

Terri, when I was in animal research we used the blood from the animal to make plasma and combined it with some thrombin from the hospital pharmacy.  Of course that is tedious especially since histogel is available.  I warmed the histogel by placing a tube of it in a beaker of hot water, do not mw it.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm at hotmail.com

From: John Garratt <john.garratt at ciqc.ca>
Sent: Thursday, January 23, 2020 10:51 AM
To: Terri Braud <tbraud at holyredeemer.com>
Cc: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Need a procedure

Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin.
Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma.
The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC.



‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Hi fellow Histonetters - I'm in need of some help, please
> Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method
> Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used?
> Thanks in advance. Histotechs rock!
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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