[Histonet] Question about gelatin embedding
jkiernan at uwo.ca
Fri Jan 24 00:01:00 CST 2020
Gelatin embedding is easy. You infiltrate the specimen and then fix it again in formaldehyde to cross-link the gelatin molecules and make the whole mass isoluble in water. You can than cut frozen sections of any kind: cryostat, or with an old-fashioned freezing microtome collecting thawed sections from the knife with a brush. The formaldehyde-fixed gelatin holds everything together. With a Nissl stain it remains inconspicuous. If you do an H&E the gelatin will stain red.
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From: Alonso Martínez Canabal via Histonet <histonet at lists.utsouthwestern.edu>
Sent: 17 January 2020 16:29
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Question about gelatin embedding
I am here again. I am wondering if someone has good experience
embedding in gelatin-albumin for cryostat or vibratome sectioning.
Specifically we use brain tissue and is common in free floating techniques
non-attached parts of the same section float around and later that
generates all sorts of problems.
Thank you very much.
Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"
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