[Histonet] Question about gelatin embedding

[John Kiernan]

Gelatin embedding is easy. You infiltrate the specimen and then fix it again in formaldehyde to cross-link the gelatin molecules and make the whole mass isoluble in water. You can than cut frozen sections of any kind: cryostat, or with an old-fashioned freezing microtome collecting thawed sections from the knife with a brush.  The formaldehyde-fixed gelatin holds everything together.  With a Nissl stain it remains inconspicuous. If you do an H&E the gelatin will stain red.
John Kiernan
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________________________________
From: Alonso Martínez Canabal via Histonet <histonet at lists.utsouthwestern.edu>
Sent: 17 January 2020 16:29
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Question about gelatin embedding

Hello,
      I am here again. I am wondering if someone has good experience
embedding in gelatin-albumin for cryostat or vibratome sectioning.
Specifically we use brain tissue and is common in free floating techniques
non-attached parts of the same section float around and later that
generates all sorts of problems.
   Thank you very much.

--
Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"
56224833
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