[Histonet] Need a procedure

[John Garratt]

Good point regarding the molecular and another good reason to move away from plasma. Plus taking plasma, even outdated plasma, is fraught with ethical and regularity problems which are best to avoid now that ever milliliter of blood product needs to be accounted for.


www.cpqa.ca

‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Thursday, January 23, 2020 3:41 PM, Garrey Faller <garreyf at gmail.com> wrote:

> Great helpful information.
> We use plasma /thrombin.
> It’s easy to use, and we have plenty of access to it from our blood bank.
>
> However, we are looking to switch to Gel.
>
> Why?
>
> 1.  If you don’t keep an eye on your plasma, at some point you could see fungi in your specimen with a chance you might think it’s real and not a contaminant. That’s a problem. Don’t keep it for too long in the refrigerator. It’s a great culture medium for fungi.
>     You could freeze small aliquots I guess.
>
> 2.  Molecular testing on cell blocks:
>     With the increasing use of molecular testing on cell blocks, plasma thrombin method poses a problem. The plasma introduces other peoples DNA into the patients sample.Think about that.
>
>     Didn’t know about the heat issue with Gel. Important to know. Thanks !
>
>     Garrey
>
>     Sent from my iPhone
>
>
> > On Jan 23, 2020, at 6:12 PM, Tony Henwood (SCHN) via Histonet histonet at lists.utsouthwestern.edu wrote:
> > Hi all,
> > Be careful of using cell block matrix that requires heat to solubilise the matrix (eg agar or other commercial matrixes like Histogel).
> > Adding a heated matrix to unfixed, or even formalin fixed, material can denature some antigens (eg CEA) resulting in a false negative IPX.
> > Unfortunately the importance of heat as a pre-analytical factor in immunohistochemistry is often not appreciated.
> > Regards
> > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> > Principal Scientist, the Children’s Hospital at Westmead
> > Adjunct Fellow, School of Medicine, University of Western Sydney
> > Tel: 612 9845 3306
> > Fax: 612 9845 3318
> > Pathology Department
> > the children's hospital at westmead
> > Cnr Hawkesbury Road and Hainsworth Street, Westmead
> > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
> > -----Original Message-----
> > From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> > Sent: Friday, 24 January 2020 8:21 AM
> > To: Muhammad Azam ajju33 at gmail.com
> > Cc: histonet at lists.utsouthwestern.edu
> > Subject: Re: [Histonet] Need a procedure
> > http://www.avantec.fr/content/dam/tfs/SDG/APD/APD Documents/Product Manuals & Specifications/Histology Equipment and Supplies/Embedding Cassettes and Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf
> > The above link will help.
> > www.cpqa.ca
> > ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
> >
> > > On Thursday, January 23, 2020 12:13 PM, Muhammad Azam ajju33 at gmail.com wrote:
> > > Anybody has validated procedure for histogel
> > > Sent from my iPhone
> > >
> > > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet histonet at lists.utsouthwestern.edu wrote:
> > > > > Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin.
> > > > > Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma.
> > > > > The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC.
> > > > > John
> > > > > www.cpqa.ca
> > > > > ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
> > > >
> > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet histonet at lists.utsouthwestern.edu wrote:
> > > > > Hi fellow Histonetters - I'm in need of some help, please
> > > > > Background - We currently use agar to capture our scant cell
> > > > > blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used?
> > > > > Thanks in advance. Histotechs rock!
> > > > > Terri L. Braud, HT(ASCP)
> > > > > Anatomic Pathology Supervisor
> > > > > Laboratory
> > > > > Holy Redeemer Hospital
> > > > > 1648 Huntingdon Pike
> > > > > Meadowbrook, PA 19046
> > > > > ph: 215-938-3689
> > > > > fax: 215-938-3874
> > > > > Care, Comfort, and Heal
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> > > >
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