[Histonet] Deparaffinized slides

[Betsy Molinari]

It does sound like fun!

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812 
Email: BMolinari at texasheart.org
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-----Original Message-----
From: Morken, Timothy <Timothy.Morken at ucsf.edu> 
Sent: Tuesday, January 21, 2020 11:32 AM
To: Betsy Molinari <BMolinari at texasheart.org>
Subject: RE: [Histonet] Deparaffinized slides

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Betsy, sounds like an NSH poster project! - do that and then try different stains after different periods of time.

But I suspect that storing them without paraffin won't be a problem. They are already fixed so nothing to degrade, except by oxidation. That means that chemical stains will probably work fine (special stains) but they might not be useful for some IHC stains after a while. What you could do, however, is just re-coat them with a thin layer of paraffin over the tissue and then I'll be they would be good for a long time (don't dip the slide - I've done that before and it makes a mess when deparaffinizing them - that is 1,000 times more wax than you usually have on a slide!).

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center


-----Original Message-----
From: Betsy Molinari via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Monday, January 20, 2020 4:56 AM
To: 'Histonet at lists.utsouthwestern.edu' <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Deparaffinized slides

Good day and Happy Martin Luther King Day. What are your thoughts on letting deparaffinized slides dry to be used for future use , or keep in DI water for a time? I deparaffinized  some slides this morning, then the researcher called and asked me to hold off on staining them. I am going to continue staining them after explaining the situation to her. But I am just curious if they can be held after being deparaffinized.
Thank you.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
Houston, Texas
832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
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