[Histonet] Histonet Digest, Vol 194, Issue 15
Betsy Molinari
BMolinari at texasheart.org
Tue Jan 21 10:00:22 CST 2020
No I did not. But I will keep that in mind if it happens again. Thanks!
Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research
Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030
Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
texasheart.org <www.texasheart.org> | facebook <www.facebook.com/Texas.Heart.Institute> | twitter <twitter.com/Texas_Heart>
-----Original Message-----
From: Rathborne, Toni <Toni.Rathborne at RWJBH.org>
Sent: Tuesday, January 21, 2020 8:56 AM
To: Betsy Molinari <BMolinari at texasheart.org>
Subject: RE: [Histonet] Histonet Digest, Vol 194, Issue 15
*** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe.
________________________________
Betsy,
I realize that they have been stained now, but did you considered running them back through alcohols and xylene and then coverslipping? The cells would then be preserved and you could easily remove the coverglass and stain at a later date.
Toni
------------------------------------------------------------------------------------
NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts.
-----Original Message-----
From: Betsy Molinari via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, January 21, 2020 7:53 AM
To: Charles Riley; 'Histonet at lists.utsouthwestern.edu'
Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15
*** This is an External Email ***
Charles, thank you for your reply. That is an interesting idea. I think I may have a bit of an experiment to run when I have some time.
Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research
Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030
Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
texasheart.org<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.texasheart.org_&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=s7JcJGstAwJMFQxH2cwi5datQ8koH8e3jHm8y1UbT_4&e= > | facebook<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.facebook.com_Texas.Heart.Institute&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=By6l4F4xTYA4WEzVCdjS8fGJqf3NfbqBtdNNUYZ6lVo&e= > | twitter<https://urldefense.proofpoint.com/v2/url?u=https-3A__twitter.com_Texas-5FHeart&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=ThGMpUv5CaIuL3kg99fGUkfvCgGe7CyppXv-cT0AFXo&e= >
From: Charles Riley <criley at dpspa.com>
Sent: Tuesday, January 21, 2020 5:55 AM
To: Betsy Molinari <BMolinari at texasheart.org>
Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15
*** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe.
________________________________
I have never done this before but I would assume you could reseal it just like you would a paraffin block by putting some fresh paraffin on top of the section again and allowing it to dry. It would just then need to be reheated and run down to remove the paraffin cover before staining.
On Tue, Jan 21, 2020 at 6:50 AM Betsy Molinari via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:
Thank you for the reply Amos. I totally agree but the researcher did not inform me until after the deparaffinization was finished. She wanted to hold off on the staining but it was too late and I went ahead with the staining. I was just curious if there was, in fact a procedure to preserve a deparaffinized slide.
Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research
Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030
Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org<mailto:BMolinari at texasheart.org>
texasheart.org<https://urldefense.proofpoint.com/v2/url?u=http-3A__texasheart.org&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=OFPer3U_R8zdgxyhat_LPh27aCF-I77Fd8s90MKKhCY&e= > <http://www.texasheart.org<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.texasheart.org&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=4WLwiQP8_wEaz7sh4N9zsKrTbyuSzxx4yfZFfVOzLQQ&e= >> | facebook <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.facebook.com_Texas.Heart.Institute&d=DwIGaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=s8850cI4Lykfzxd1w93MHExc6zBsdDg1D415DkfU60s&s=x6BxN53d8RbdCJ2nQrv5A5jq-pMRaRHCCBhwGdARATs&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.facebook.com_Texas.Heart.Institute&d=DwMFaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=5wwoBF-rqq3qoavhQKMOdIzTCUln4iHFsjxxPGR9Bfo&s=1j35cGOcpS8XTF-s3iJ8uygoX2GwJATdlan6K30kVBc&e=>> | twitter <twitter.com/Texas_Heart<https://urldefense.proofpoint.com/v2/url?u=http-3A__twitter.com_Texas-5FHeart&d=DwMFaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=5wwoBF-rqq3qoavhQKMOdIzTCUln4iHFsjxxPGR9Bfo&s=bAt2vap3IqD4Rg9oUf2THqKjQg1_-BqxwaZPWbaUi_A&e=>>
-----Original Message-----
From: Amos Brooks via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Sent: Monday, January 20, 2020 5:59 PM
To: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15
*** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe.
________________________________
Hi Betsy,
I wouldn't do it. It's an unnecessary risk to let them dry out. Better to leave it in distilled water if you absolutely must. Ideal to not deparaffinize in the first place if you can't finish the stain. Dried out sections is just a bad plan.
Amos Brooks
On Mon, Jan 20, 2020, 1:00 PM <h-
>
> Message: 1
> Date: Mon, 20 Jan 2020 12:56:20 +0000
> From: Betsy Molinari
> <BMolinari at texasheart.org<mailto:BMolinari at texasheart.org>>
> To: "'Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu>'"
>
> <Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwester
> n.edu>>
> Subject: [Histonet] Deparaffinized slides
> Message-ID:
> <
> SN6PR10MB28955C0F9DCD3FE5C5FDF3FDCE320 at SN6PR10MB2895.namprd10.prod.out
> <mailto:SN6PR10MB28955C0F9DCD3FE5C5FDF3FDCE320 at SN6PR10MB2895.namprd10.
> prod.out>
> look.com<https://urldefense.proofpoint.com/v2/url?u=http-3A__look.com&
> d=DwMFaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJ
> LzefN3bjagdyUrCioVawjbCC16NNH8&m=5wwoBF-rqq3qoavhQKMOdIzTCUln4iHFsjxxP
> GR9Bfo&s=A65vpkkkXUawRC83YPizUWdZcJI2-rmtq1qxbdZHobM&e=>
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Good day and Happy Martin Luther King Day. What are your thoughts on
> letting deparaffinized slides dry to be used for future use , or keep
> in DI water for a time? I deparaffinized some slides this morning,
> then the researcher called and asked me to hold off on staining them.
> I am going to continue staining them after explaining the situation to
> her. But I am just curious if they can be held after being deparaffinized.
> Thank you.
>
> Betsy Molinari HT(ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> Houston, Texas
>
*****************************************
>
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu>
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=GcQ8xaDLGa-5zMPbj3K35pahQ08SV4dj5Ka_AOrT2sc&s=WShxiyrJyy3oGjhwV0jxCmtB0sXFvS1Q0rBKL4u_bRI&e=
This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu>
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=GgB_0QqIO1phGyhrsy0t5-VgXZcH1YaAdKAlsXERdO8&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwMFaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=5wwoBF-rqq3qoavhQKMOdIzTCUln4iHFsjxxPGR9Bfo&s=hfi0Ln3CE6JLpD89RXSyUxh9GtCa6bT6N-ZIe6L1FqE&e=>
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=GgB_0QqIO1phGyhrsy0t5-VgXZcH1YaAdKAlsXERdO8&e=
More information about the Histonet
mailing list