[Histonet] X-gal staining

[H. Etchevers]

I agree with Carl about the X-gal activity being destroyed by processing 
for paraffin sections (I'm a researcher, not a histologist).

However, in our hands the anti-b-galactosidase antibody, reference 
A-11132, from ThermoFisher DOES work on paraffin sections (many others 
don't). It has been published with subsequent incubation with a 
biotinylated secondary (Vector BA-1000) and standard procedures 
thereafter to show good signal with DAB. We do immunofluorescence.

MPBio used to sell a good polyclonal for this purpose, but it has since 
been discontinued.

Best wishes,

Heather

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*Marseille Medical Genetics Centre 
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*Heather C. Etchevers, Ph.D.*

*MMG - INSERM U1251*
Faculté de Médecine AMU
27 boulevard Jean Moulin
13005 Marseille - France

Tel: +33(0)4 91 32 49 37
heather.etchevers at inserm.fr <mailto:heather.etchevers at inserm.fr>

	

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