[Histonet] IHC Troubleshooting and Water Baths

[Nancy Schmitt]

Hi Charles-

IHC staining - We also use the Leica Bond staining system and had tissue fall off in the beginning.  We blot the slides to remove any additional water and then place in drying oven for 30 minutes at 60 before placing on the stainer.

Water baths - at the end of each day the water is dumped, the bath is rinsed with bleach solution, rinsed with water and dried.

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager


-----Original Message-----
From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, February 26, 2020 9:44 AM
To: Histo List
Subject: [Histonet] IHC troubleshooting

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Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining.

Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process?     We use the Leica Bond stainers.

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs


Message: 5
Date: Wed, 26 Feb 2020 17:09:14 -0500
From: Charles Riley <criley at dpspa.com>
To: Histo List <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Poll question
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How often does everyone clean their water-bath?

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs