[Histonet] IHC troubleshooting

[Cartun, Richard]

Are you using some form of antigen retrieval?  In addition to what has been discussed previously, antigen retrieval using heat (HIER) can cause tissue loss especially collagen.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
Richard.cartun at hhchealth.org

-----Original Message-----
From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, February 26, 2020 9:44 AM
To: Histo List
Subject: [Histonet] IHC troubleshooting

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Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining.

Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process?     We use the Leica Bond stainers.

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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