[Histonet] IHC troubleshooting

[Abdulbasit Andijany]

In my experience;
If the the specimen is under-fixed or under-processed, the section will be sloughed off during IHC staining.

I find proper fixation time and drying the slides at room temperature (before baking them in oven) optimal.

Andy



Abdulbasit Andijany (Andy)
AMLS, BBMS, MSc, CT (ASCP)CM, HTL (ASCPI)
King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia


On 26 Feb 2020, at 18:35, John Garratt via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:

Do you float and pick up your sections from a water bath of distilled water with no adhesive added? If not, doing that will help.

John

On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:

Our pathologists are complaining that chunks of the dermis are missing from
IHC slides yet the entire section is present prior to staining.

Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process? We use the Leica Bond stainers.

--

Charles Riley BS HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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