[Histonet] overstained Warthin starry

[Terri Braud]

Any silver stains can be lightened or removed by the old method for removing mercury pigment.  This particular timing will remove it all so I would start the sequence of steps in 5 second increments, repeat or lengthen times as needed.
1. Hydrate to distilled water.
2.Place in Lugols iodine for 5 minutes.
3.Rinse in two changes of distilled water.
4.Place in 2% sodium thiosulfate for 1 minute.
5.Wash in running tap water and rinse in two changes of distilled water.


Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Message: 2
Date: Tue, 11 Feb 2020 10:33:12 -0500
From: Charles Riley <criley at dpspa.com>
To: Histo List <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Warthin starry
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	<CAAQhB117ytgHO773Z0z-72ZDH0ac-7SzBt_h7nnhDtzFjiix1g at mail.gmail.com>
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Is there any way to lighten and over stainer WS stain?

Charles Riley BS  HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs