[Histonet] Detection systems for IHC's

[Joe Myers]

Charles:
Since the literature for BioGenex’s detection reagents for their Xmatrx Infinity system (XvisTM) indicates use of an ‘enhancing’ reagent prior to application of the (tertiary) antibody/polymer/enzyme reagent, such a detection system would be defined as a ‘two-step’ method.  In contrast, Leica’s Refine reagent is a one-step reagent/method.  Generally speaking, two-step methods are usually more sensitive than one-step methods, but since I’ve not conducted a comparison of the results obtained with these quite different reagents/methods, I can’t offer any advice on which is actually ‘better’.  I hope you find this feedback useful.
Cheers,
Joe Myers, M.S., CT/QIHC(ASCP)

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Message: 1
Date: Mon, 21 Dec 2020 18:57:57
From: Charles Riley <criley at dpspa.com>
To: "histonet at lists.utsouthwestern.edu"
   <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Detection systems for IHC's
DOes anyone know what the comparison detection kit for the Xfinity Matrix from Biogenex would be in relation to the Bond Refine detection kits?