[Histonet] human shoulder joint fixation

[Hobbs, Carl]

Jeez....a human shoulder joint?
Be grateful for background.
Many years ago I had to fix/process to Polyester resin many monkey condylar joints ( jaw)
As iterated  here, I stripped all superfluous tissue off and processed the joints to resin.
( as I do now when doing Histology on ms knee joints as model for arthritis....but, using Pwax after decal)
 Monkey mandibles:  fix in Formalin...on a rocker for 2 wks ( 20:1 Formalin:PBS)
Changed twice
I then took them specimens to a dedicated lab who sectioned this resin using a diamond circular saw.
I was too young to follow the process further...also, in them days , Technicians did what they were told and were never appreciated.
Maybe a different fix fluid will give a better morphology....JK will advise, surely!
However, the project I was involved with won a prize...somewhere ( too young to be bothered)

C







Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 


020 7848 6813