[Histonet] Histonet Digest, Vol 201, Issue 3
Madeleine Huey
madeleinehuey at gmail.com
Wed Aug 5 16:08:10 CDT 2020
Date: Wed, 5 Aug 2020 15:34:28 +0000
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Cytology
Message-ID: <903be99abb344593a73079761d76299b at PHX-EXCH-013.chw.edu>
Content-Type: text/plain; charset="us-ascii"
Good Morning,
I rarely do Special stains or IHC's on Cytology cytospins or thin prep type
slides. Should these slides be charged, air dried or fixed in 95%
alcohol before doing Special stains or IHC's on them?
Is there a good book or some sort of publication on cytology procedures
regarding the above mentioned. I have tried and look it up and it seems
all over the place on what to do.
Any help would be greatly appreciated.
Hello Karen,
Long time no see! I always used Charged slides, and prefixed Methanol for
~ 5 min @ RT (not 95% alcohol) before staining (SS & IHC).
Hope this helps!
*Madeleine Huey, B.S., HTL & QIHC (ASCP)*
Supervisor, Pathology/Histology & IPOX (MV & LG)
El Camino Hospital
2500 Grant Road (Rm GC-33)
Mountain View, CA 94040
Phone: 650-940-7038
Fax: 650-988-8387
Email: madeleine_h at elcaminohospital.org
On Wed, Aug 5, 2020 at 10:14 AM <histonet-request at lists.utsouthwestern.edu>
wrote:
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> Today's Topics:
>
> 1. process formalin-fixed tissues from animals infected with a
> virus (Amy Lee)
> 2. Update info. on old topic?
> (Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP))
> 3. Re: Update info. on old topic? (Paula Keene Pierce)
> 4. MMR IHC validaton (Waitts, Celeste)
> 5. Cytology (Heckford, Karen - SMMC-SF)
> 6. Re: Update info. on old topic? (Morken, Timothy)
> 7. Exciting New Histology Opportunities!! (Pam Barker)
> 8. Quick question about Florida licensure. (Pam Barker)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 4 Aug 2020 11:13:42 -0700
> From: Amy Lee <amyleehisto779 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] process formalin-fixed tissues from animals
> infected with a virus
> Message-ID:
> <CAGnbBwBxD9+uK7LyTdXVVBz0EGVcciTTR=
> OdXM+2Y9GnBC+40g at mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Hello,
> Does anyone know what we should know about process formalin fixed animal
> tissue that has been infected with virus? What
> certificate/requirement/qualification we should have? We have never done
> this before and need to make decision if we should do it or not.
>
> Thank you very much,
>
> Amy
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 5 Aug 2020 12:39:53 +0000
> From: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" <jqb7 at cdc.gov>
> To: "'histonet at lists.utsouthwestern.edu'"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Update info. on old topic?
> Message-ID:
> <
> SA9PR09MB5325CBDE76121B31C6309F23804B0 at SA9PR09MB5325.namprd09.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Good morning!
>
> I need to provide some information to my team about film vs. glass
> coverslipping. I have a lot of positive info. regarding film but are any
> cons? And I need to come up wit the pros of glass over film. Anyone with
> extended experience have some current information for me?
>
> Thanks very much,
>
> Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP)
> Centers for Diseases Control and Prevention
> 1600 Clifton Road NE
> MS H18-SB
> Bldg. 18, Rm SB-114
> Atlanta, GA 30329
> 404-639-3590
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 5 Aug 2020 13:00:37 +0000 (UTC)
> From: Paula Keene Pierce <paula at excaliburpathology.com>
> To: "'histonet at lists.utsouthwestern.edu'"
> <histonet at lists.utsouthwestern.edu>, "Sanders, Jeanine
> (CDC/DDID/NCEZID/DHCPP)" <jqb7 at cdc.gov>
> Subject: Re: [Histonet] Update info. on old topic?
> Message-ID: <1398404991.167739.1596632437483 at mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
> I am interested in this too, with my main question being the quality of
> digital images from film coverslipped slides.
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830
> N Blue Lake DriveNorman, OK 73069PH 405-759-3953
> http://www.excaliburpathology.com
>
> A sharp knife is nothing without a sharp eye. - Klingon Proverb
>
> On Wednesday, August 5, 2020, 07:57:05 AM CDT, Sanders, Jeanine
> (CDC/DDID/NCEZID/DHCPP) via Histonet <histonet at lists.utsouthwestern.edu>
> wrote:
>
> Good morning!
>
> I need to provide some information to my team about film vs. glass
> coverslipping. I have a lot of positive info. regarding film but are any
> cons? And I need to come up wit the pros of glass over film. Anyone with
> extended experience have some current information for me?
>
> Thanks very much,
>
> Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP)
> Centers for Diseases Control and Prevention
> 1600 Clifton Road NE
> MS H18-SB
> Bldg. 18, Rm SB-114
> Atlanta, GA 30329
> 404-639-3590
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 5 Aug 2020 15:22:48 +0000
> From: "Waitts, Celeste" <CWaitts at Centrastate.com>
> To: "'histonet at lists.utsouthwestern.edu'"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] MMR IHC validaton
> Message-ID:
> <8c30fb230afd42f7a54c520887028bc2 at exchnode1.local.centrastate.com>
> Content-Type: text/plain; charset="us-ascii"
>
> HI,
> WHAT is everyone's opinion on the best way to validate the Roche MMR IHC
> panel. Cost, time, ease?
> Any help?
> Thank you
> Celeste Waitts
> Histology Supervisor
> Centrastate Medical Center
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 5 Aug 2020 15:34:28 +0000
> From: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>
> To: "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Cytology
> Message-ID: <903be99abb344593a73079761d76299b at PHX-EXCH-013.chw.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Morning,
> I rarely do Special stains or IHC's on Cytology cytospins or thin prep
> type slides. Should these slides be charged, air dried or fixed in 95%
> alcohol before doing Special stains or IHC's on them?
>
> Is there a good book or some sort of publication on cytology procedures
> regarding the above mentioned. I have tried and look it up and it seems
> all over the place on what to do.
>
> Any help would be greatly appreciated,
>
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckford at dignityhealth.org<mailto:karen.heckford at dignityhealth.org>
>
> Caution: This email message, including all content and attachments, is
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>
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 5 Aug 2020 15:38:14 +0000
> From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
> To: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" <jqb7 at cdc.gov>
> Cc: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Update info. on old topic?
> Message-ID:
> <
> BYAPR05MB57039195DFE1E6C67B67B1F7E74B0 at BYAPR05MB5703.namprd05.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Jeanine!
>
> We are using film coverslipping exclusively now and scanning all slides.
> We moved to film because it dries almost instantly and can be scanned right
> away. It has worked very well. The only issues, and not specific to film
> coverslipping, are that some slides with low contrast sometimes are out of
> focus. That is primarily IHC slides with low contrast counter stain and low
> contrast specific staining.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
> -----Original Message-----
> From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet <
> histonet at lists.utsouthwestern.edu>
> Sent: Wednesday, August 05, 2020 5:40 AM
> To: 'histonet at lists.utsouthwestern.edu' <histonet at lists.utsouthwestern.edu
> >
> Subject: [Histonet] Update info. on old topic?
>
> Good morning!
>
> I need to provide some information to my team about film vs. glass
> coverslipping. I have a lot of positive info. regarding film but are any
> cons? And I need to come up wit the pros of glass over film. Anyone with
> extended experience have some current information for me?
>
> Thanks very much,
>
> Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) Centers for Diseases Control
> and Prevention
> 1600 Clifton Road NE
> MS H18-SB
> Bldg. 18, Rm SB-114
> Atlanta, GA 30329
> 404-639-3590
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
>
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=rzbMGLf4Y17ghrmD701AEURtk3lAZ5QHWvK_mUBrhTs&s=NdAh9f9sPJR8Cc0amnB3m1WH4qmQ7DH1q5uQoy7e2k4&e=
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 5 Aug 2020 12:14:21 -0400
> From: "Pam Barker" <relia1 at earthlink.net>
> To: "Histopeeps Histonet" <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Exciting New Histology Opportunities!!
> Message-ID: <000001d66b43$7b758ad0$7260a070$@earthlink.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello Histonetters,
> I hope you are having a great week.
> I wanted to send a quick note to tell you about the positions that I am
> working on and am most excited about.?
> Why am I excited about these positions?
> Because each of these clients was asked if I had someone for you today
> would
> you be ready to interview and hire?
> Every One of these clients said YES!!!
> Here are the Super Sizzlin Hot Opportunities!
>
> Leadership Opportunities:
> North Carolina ? Greensboro
> North Carolina - Concord
>
> Histology Tech, IHC and Mohs Tech Opportunities
> N. Carolina Charlotte
> N. Carolina Wilmington ? Mohs
> N. Carolina Wilmington
> Florida Sarasota area
> California - Modesto ? IHC
> California Modesto
>
> All of these positions are full time and permanent!!
> My clients offer excellent compensation, benefits and in most cases either
> relocation or a sign-on bonus.
>
>
> Histopeeps, If you think you or someone you know might be interested in any
> of these opportunities or would like to talk about a job search in another
> area, please contact me.
> If I place someone you refer You will earn a referral fee.
>
> If you are interested in any of these opportunities
> CALL /TEXT MY CELL ASAP!!! At 407-353-5070.
>
>
> I can also be reached toll free at the office at 866-607-3542 or at
> relia1 at earthlink.net to set up a time to talk at your convenience!
>
> Thanks-Pam
>
> Right Time, Right Place, Right Move with RELIA!
> *15 Years!*
> Celebrating 15 years of service exclusively to the Histology Community!
>
> Thank You!
> ?Pam M. Barker?
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell:???? (407)353-5070
> FAX:???? (407)678-2788
> E-mail: relia1 at earthlink.net
> https://www.facebook.com/RELIASolutionsforhistologyprofessionals
> www.facebook.com/PamBarkerRELIA
> www.linkedin.com/in/reliasolutions
> www.twitter.com/pamatrelia
> check out our latest opportunities at:
> http://www.jobvertise.com/members/relia1
> #jobs4myhistopeeps
> #ilovemyhistopeeps
> #histopeeps
> Follow my hashtags and make your day great and your career greater!!
>
>
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 5 Aug 2020 12:19:28 -0400
> From: "Pam Barker" <relia1 at earthlink.net>
> To: "Histopeeps Histonet" <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Quick question about Florida licensure.
> Message-ID: <001701d66b44$323b8d00$96b2a700$@earthlink.net>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Histopeeps!
> I have a quick question about Florida licensure. Can someone who holds a
> MLT(ASCP) get licensed and work as a histotech in Florida?
>
> Thanks-Pam
>
> Right Time, Right Place, Right Move with RELIA!
> *15 Years!*
> Celebrating 15 years of service exclusively to the Histology Community!
>
> Thank You!
> Pam M. Barker
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: relia1 at earthlink.net
> https://www.facebook.com/RELIASolutionsforhistologyprofessionals
> www.facebook.com/PamBarkerRELIA
> www.linkedin.com/in/reliasolutions
> www.twitter.com/pamatrelia
> check out our latest opportunities at:
> http://www.jobvertise.com/members/relia1
> #jobs4myhistopeeps
> #ilovemyhistopeeps
> #histopeeps
> Follow my hashtags and make your day great and your career greater!!
>
>
>
>
> ------------------------------
>
> Subject: Digest Footer
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> End of Histonet Digest, Vol 201, Issue 3
> ****************************************
>
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