[Histonet] linear stainer protocol

[Terri Braud]

We have the Leica linear stainer (Also sold by Avantik?) and have it set at 7 second intervals. The stain takes about 100 seconds. 
Slides are fixed in the Coplin jar with 95% Alcohol then loaded:
The stations are in order:
1. Water wash
2. Hemotoxylin (Richard Allen 7211)
3. Hemotoxylin (Richard Allen 7211)
4. Hemotoxylin (Richard Allen 7211)
5. Water wash
6. Bluing (we use Surgipath SelecTech Blue Buffer 8 concentrate, diluted according to directions
7. Water wash
8. Eosin (1% Alcoholic)
9. 95% Alcohol
10. Absolute Alcohol
11. Absolute Alcohol
12. Absolute Alcohol
13. Xylene
14. Xylene

The slides are clean and consistent with great differentiation.  The Richard Allen 7211 Hematoxylin does not require filtering and seems to last forever.
I sincerely hope this helps.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Today's Topics:

   1. Routine H&E frozen section protocol (Raible, John C.)
   2. Re: Routine H&E frozen section protocol (Porter, Amy)
   3. FFPE PRC testing (Charles Riley)


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Message: 1
Date: Fri, 24 Apr 2020 12:10:26 +0000
From: "Raible, John C." <John.Raible at va.gov>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Routine H&E frozen section protocol
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Hello Histonet,

My name is John Raible, and I work at the VA medical center in Minneapolis. I am trying to find routine H&E frozen section staining protocols for an automated linear stainer. My lab recently got one, and I was asked to research staining protocols Any help you could provide would be greatly appreciated.

Thank you all. Stay safe and healthy!

John


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Message: 2
Date: Fri, 24 Apr 2020 12:26:28 +0000
From: "Porter, Amy" <portera at msu.edu>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>,	"Raible, John C."
	<John.Raible at va.gov>
Subject: Re: [Histonet] Routine H&E frozen section protocol
Message-ID:
	<CH2PR12MB4230567AFE1B34E45A537347A4D00 at CH2PR12MB4230.namprd12.prod.outlook.com>
	
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Fix in 10% NBF - 10 minutes
Rinse in running tap water - 5 minute
Rinse in distilled water
Gill 2 Hematoxylin - 1 1/2 minutes
1% Glacial Acetic water - 3 dips
Running water - 2 minutes
(we do not need bluing agent - our water takes care of it)
95% Ethanol -2 minutes
1% Eosin/Phloxine - 2 minutes
95% Ethanol - 2 minutes
Remaining dehydration in absolute ethanol & xylene - 2 mintues rest of the way down



________________________________
From: Raible, John C. via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Friday, April 24, 2020 8:10 AM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Routine H&E frozen section protocol

Hello Histonet,

My name is John Raible, and I work at the VA medical center in Minneapolis. I am trying to find routine H&E frozen section staining protocols for an automated linear stainer. My lab recently got one, and I was asked to research staining protocols Any help you could provide would be greatly appreciated.

Thank you all. Stay safe and healthy!

John
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Message: 3
Date: Fri, 24 Apr 2020 12:13:58 -0400
From: Charles Riley <criley at dpspa.com>
To: Histo List <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] FFPE PRC testing
Message-ID:
	<CAAQhB13qr=1NOikTRh07Qm0ot0Qb3oVsLajw97HsTQKjKYZvmg at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

What platform does everyone use to carry out FFPE PCR testing?

Seeking to bring in as many genetic mutation testing options as possible
(BRAF, KRAS, NRAS...etc.)



-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs


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End of Histonet Digest, Vol 197, Issue 22
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