[Histonet] Recommended thickness of Amyloid sections

[Morken, Timothy]

Ken, yes, 8 to 10 um. The extra thickness make the bi-refringence under polarized light brighter. Also the deposits can be variable so even with the light microscope the reddish deposits will stain stronger with thicker sections. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-----Original Message-----
From: Ken M via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Thursday, April 16, 2020 1:06 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Recommended thickness of Amyloid sections

Hello All.  We have always cut all of our histology control slides at 5m.  We were told today that it is common practice to cut Amyloid at 8m?  Is this your experience?

Ken
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