[Histonet] Histonet Digest, Vol 197, Issue 8

John Garratt john.garratt at ciqc.ca
Tue Apr 14 12:36:50 CDT 2020 

Good morning, please take a look at one of the EQA reports (example in the link below) for MMR from the Canadian Pathology Quality Assurance programme. (www.cpqa.ca) .
You cannot say MMR is positive or negative since it is confusing but say if there is Expression or Absence of Expression of a MMR protein. Fortunately tissues come with their own internal "positive" and "negative" controls which you can utilise. You could design a verification program around the presence or absence of a particular MMR protein should you wish.

http://cpqa.ca/assessments/Run%2096%20MMR%20Summary.pdf

Should you wish to receive our technically useful EQA reports in your mailbox email subscriptions at cpqa.ca putting SUBSCRIBE in the subject box.

John


www.cpqa.ca

‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Tuesday, April 14, 2020 6:54 AM, Dionee Neamand via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Hello. I am looking for help with IHC staining. Does anyone have good negative control tissue for MMR stains? We need a minimum of 10 negative results per IHC stain for our validation. We have yet to find any. Any suggestions would be helpful.
> Thanks,Dee
> Dionee Neamand, HT (ASCP)Lead TechEvangelical Community HospitalLewisburg  PA  17837
>
> -----Original Message-----
> To: histonet at lists.utsouthwestern.edu
> Sent: Thu, Apr 9, 2020 1:00 pm
> Subject: Histonet Digest, Vol 197, Issue 8
>
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> Today's Topics:
>
>   1. Question concerning H-Pylori Staining (Ken M)
>   2. Is there anything I can do for you? (Pam Barker)
>   3. Competency questionnaires (Charles Riley)
>
>
> --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 8 Apr 2020 19:50:09 +0000
> From: Ken M kdean70 at hotmail.com
> To: Histo List histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Question concerning H-Pylori Staining
> Message-ID:
>     SN6PR06MB43343F3F0C62CBA60BDE54C8ADC00 at SN6PR06MB4334.namprd06.prod.outlook.com
>
>    
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi everyone!  We have a question about staining for H-Pylori Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue stock, Sodium Hydroxide) we notice what clearly  looks like the H-Pylori purple stained clusters, but after dehydration in 100% alcohol the purple clusters seem to disappear. Should we just dehydrate using a slide oven instead of the alcohol? For how long at what temp? Could the alcohol be affecting the purple color making it too light to see?
>
> Michelle
>
>
> -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>
> Message: 2
> Date: Thu, 9 Apr 2020 11:15:47 -0400
> From: "Pam Barker" relia1 at earthlink.net
> To: "Histopeeps Histonet" histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Is there anything I can do for you?
> Message-ID: 008901d60e81$bf898880$3e9c9980$@earthlink.net
>
> Content-Type: text/plain;    charset="iso-8859-1"
>
> Hi Histonetters,
> I hope you and your loved ones are staying safe and healthy, and you can
> take time to enjoy Easter, Passover  and this season with your family.
> It seems that things are changing on an almost daily basis. 
> I have been monitoring social media - personal connections and histology
> related groups on Facebook and LinkedIN and have been staying in touch with
> many of my active candidates and customers.
> I have gotten a lot of questions about what is going on with the ?career?
> aspect of histology at this time.
> Here is a summary of what I have observed and experienced so far:
>
> Histotechs supporting ?non-essential surgeries? i.e. Derm and GI private
> practices:
> ? Some are experiencing cuts in hours and/or are using the time to catch up
> on backlogs and lab maintenance.
> ? Many are being furloughed with the expectation of being called back to
> work once ?the all clear is given?,
> ? Start dates for new employees are being postponed until further notice.
>
> Traveling Histotechs - Histotechs that are working as contractors
> nationwide:
> ? Most of the travel techs I have heard from have been grounded meaning sent
> home until further notice.
> ? They seem confident that they will be returning to their assignments as
> they are looking for prn positions for now.
>
> Hospital based histotechs:
> ? Some are being furloughed
> ? Some are having their hours reduced
> ? Some are job sharing meaning for example if they have a regular staff of 4
> techs they take turns 2 at a time working for a week while the other 2 stay
> home
> ? Some are being used in other areas for example if they have experience in
> phlebotomy they are moving to that department.
>
> I can?t really speak to research, academic or pharmaceutical areas as I
> haven?t heard much from them.  (Hopefully that?s because they are busy
> working on the COVID-19 response.)
>
> What I glean from this and bear in mind this only an opinion on my part
> based on what I have heard anecdotally.  It seems that the best case
> scenario at this point is there was a shortage of histotechs before the
> pandemic and there will be just as great a need after the pandemic in other
> words we are just pressing pause.
>
> Just Remember?
> Things Will Get Better. 
> This Will End.
> &
> We Will Return To Our Normal Lives.
>
> In the meantime?
> Is there anything I can do for you?
> Were you actively looking for a new position before this crisis?
> Are you someone who is unemployed as opposed to furloughed?
> Are you feeling lonely or isolated and just need to hear a friendly voice?
> Call Me,
> Text Me,
> Email Me,
> Or Contact Me through Social Media.
>
> We are all in this together?
>
> Looking forward to the light at the end of the tunnel.
>
> Thanks-Pam
>
> Right Time, Right Place, Right Move with RELIA!
> 15 Years!
> Celebrating 15 years of service exclusively to the Histology Community!
>
> Thank You!
> ?Pam M. Barker?
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell:???? (407)353-5070
> FAX:???? (407)678-2788
> E-mail: relia1 at earthlink.net 
> https://www.facebook.com/RELIASolutionsforhistologyprofessionals
> www.facebook.com/PamBarkerRELIA
> www.linkedin.com/in/reliasolutions
> www.twitter.com/pamatrelia 
> check out our latest opportunities at:
> http://www.jobvertise.com/members/relia1
> #jobs4myhistopeeps
> #ilovemyhistopeeps
> #histopeeps
> Follow my hashtags and make your day great and your career greater!!
>
>
> ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>
> Message: 3
> Date: Thu, 9 Apr 2020 12:47:44 -0400
> From: Charles Riley criley at dpspa.com
> To: Histo List histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Competency questionnaires
> Message-ID:
>     CAAQhB10D2e=CMBZD6nqGcrX19sWQneBOnYZy1-ijTfb7yu-XOA at mail.gmail.com
>
> Content-Type: text/plain; charset="UTF-8"
>
> Does anyone use a questionnaire for testing a techs competency either by
> itself or on top of practical application of the skills needed?  If so
> would you mind sharing with me your questions (I am looking for grossing,
> microtomy and troubleshooting, embedding, processing, and
> immunohistochemistry/special/H&E staining and troubleshooting.
>
> ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>
> Charles Riley BS  HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
>
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