[Histonet] Reference website RE: Flammable Sprays in Cryostats

Thu Sep 26 10:29:35 CDT 2019

You can see past issues of Morbidly and Mortality Weekly Report (MMWR) at:

https://www.cdc.gov/mmwr/index2019.html

For past issues, click on "weekly report" on the left hand side and you can get to a list of past issues.

However, for the publication about cryostats, look under "Supplements" a bit lower on the page and choose the 2012 supplement issue (Vol 61) . It is in the one on Laboratory Safety. It has a lot more than just cryostat safety.

https://www.cdc.gov/mmwr/preview/ind2012_su.html

Scroll to the very bottom of the page to see the January supplement

The full reference is:

MMWR January 6, 2012 / Vol. 61 / Supplement / Pg. 1 - 105

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-----Original Message-----
From: Sheeder, Christopher via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, September 26, 2019 8:00 AM
To: jasonhauser71 at gmail.com; P Sicurello
Cc: Histonet
Subject: Re: [Histonet] Flammable Sprays in Cryostats

I would like the link as well, if possible.

Christopher Sheeder, HT(ASCP)QIHC
Histology Supervisor | Department of Laboratories
Seattle Children’s Hospital
4800 Sand Point Way NE
Seattle, WA 98105

-----Original Message-----
From: jasonhauser71 at gmail.com <jasonhauser71 at gmail.com>
Sent: Thursday, September 26, 2019 6:01 AM
To: P Sicurello <patpxs at gmail.com>
Cc: Akemi <akemiat3377 at gmail.com>; Knutson, Deanne <DKnutson at primecare.org>; Histonet <Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Flammable Sprays in Cryostats

I woukd like the link to that. I looked around a bit on the web but couldnt locate usinf search.Jason HauserSenior HistotechnologistThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Thu, Sep 26, 2019 7:56 AMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsThis was copied from the Centers for Disease Control's Morbidity and Mortality Weekly Report (January 2012)5.9.2. Work at the open benchFrozen sections— Frozen sectioning is performed on fresh tissue and is a high-risk procedure for infectious exposure. Freezing tissue does not kill organisms, and the use of the cryostat cutting blade creates potentially dangerous aerosols. Discuss the true clinical necessity for frozen sectioning with the surgical team.— Although some cryostat instruments have a downdraft into the instrument, aerosols are dispersed into the room where the cutting takes place. Do not use freezing propellant sprays, which speed the freezing process by a few seconds and cause aerosolization of not only the tissue being frozen but also the tissues from previously cut specimens that are at the base of the instrument. Such procedures generate aerosol and droplet contamination, posing an infectious risk to all personnel in the area (56,79,82). The Clinical and Laboratory Standards Institute and others have recommended discontinuation of freezing sprays because they are not recommended by the manufacturers of cryostat instrumentation (2,79).  Sincerely,

Paula
Sicurello, HTL (ASCP)CM

Histotechnology
Specialist

UC San Diego Health

9300 Campus Point DriveLa Jolla, CA 92037

(P):
858-249-5610

Confidentiality Notice: The
information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.  Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.  If you received this e-mail in error, please contact the sender and delete the material from any computer.On Thu, Sep 26, 2019 at 4:37 AM jasonhauser71 at gmail.com <jasonhauser71 at gmail.com> wrote:

    I have heard this before. Any documention of this actually happening.I have tested for blood on surfaces outside the cabinet and never observed a positive result.Jason HauserSenior HistotechThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Wed, Sep 25, 2019 11:17 PMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsIt's not safe to use any type of spray in a cryostat.  It creates aerosols of who knows what (tuberculosis) from potentially infectious patient biopsies.  I would not recommend it, just as a universal precaution.Sincerely,

Paula
Sicurello, HTL (ASCP)CM

Histotechnology
Specialist

UC San Diego Health

9300 Campus Point DriveLa Jolla, CA 92037

(P):
858-249-5610

Confidentiality Notice: The
information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.  Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.  If you received this e-mail in error, please contact the sender and delete the material from any computer.On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet <histonet at lists.utsouthwestern.edu> wrote:There are non flammable sprays available. We got the recall notice and switched our old inventory out. I found 3 different histology specific sprays. The electronic industry offers some as well. Another option is just using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding.

Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet  wrote:
>
> I just received a letter from Leica Biosystems where they are
> prohibiting the usage of flammable freezing sprays in their cryostats.  What are others using in their cryostats to instantly freeze specimens?
>
> Thank you.
>
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknutson at primecare.org
>
>
>
>
>
>
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