[Histonet] Flammable Sprays in Cryostats

[jasonhauser71 at gmail.com]

I have heard this before. Any documention of this actually happening.I have tested for blood on surfaces outside the cabinet and never observed a positive result.Jason HauserSenior HistotechThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Wed, Sep 25, 2019 11:17 PMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsIt's not safe to use any type of spray in a cryostat.  It creates aerosols of who knows what (tuberculosis) from potentially infectious patient biopsies.  I would not recommend it, just as a universal precaution.Sincerely,

Paula
Sicurello, HTL (ASCP)CM

Histotechnology
Specialist

UC San Diego Health 

9300 Campus Point DriveLa Jolla, CA 92037

(P):
858-249-5610

 

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computer.On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet <histonet at lists.utsouthwestern.edu> wrote:There are non flammable sprays available. We got the recall notice and switched our old inventory out. I found 3 different histology specific sprays. The electronic industry offers some as well. Another option is just using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding.

Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet  wrote:
> 
> I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable
> freezing sprays in their cryostats.  What are others using in their cryostats to instantly freeze specimens?
> 
> Thank you.
> 
> 
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknutson at primecare.org
> 
> 
> 
> 
> 
> 
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