[Histonet] How do you section PPFE tissue in embedding rings?

[Paula Keene Pierce]

Hi,
I used embedding rings back in the 80s and still use the chuck adapter for large blocks. I do eyes.
I have a couple John's Hopkins chuck adapters and could loan you one.

Send me a picture of your microtome with a closeup of the chuck adapter to see if it will fit.
Do you have an old AO 820 sitting around? I know it will fit it.
The old Leica 1512s had an adapter that would work too.
Otherwise, I suggest just re-embedding.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

    On Thursday, September 5, 2019, 01:16:21 PM CDT, Preiszner, Johanna via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Hi,

I received a lot of specimens embedded in plastic embedding rings...have a Leica RM2135 microtome.

How can I put these blocks in the clamps of the microtome?

Searched Internet with zero success.

Nobody in town ever used embedding rings so I can not borrrow an adapter if that's what I need.

Could anybody please help out?

Re-embedding the tissue in regular cassettes would cost the client a lot of money.


Thanks,

Johanna Preiszner
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