[Histonet] Reprocessing tissue

Tue Sep 3 16:20:50 CDT 2019

There are several papers on this. We did a purposeful underprocess and cut and stained it, then reprocessed as normal and compared. The results were eye-opening in terms of how good it worked, but you need to factor in that the second processing will diminish IHC staining quality.

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________________________________
From: Cassie P. Davis via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, September 3, 2019 1:54:13 PM
To: Perl , Alison <aperl at caremount.com>
Cc: 'Cassie P. Davis via Histonet' <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Reprocessing tissue

Thank to those who responded so quickly. It happens maybe once a quarter. It happened today because the programs got switched: large tissue processed on biopsy program. I've heard of folks doing it this way with the theroy the paraffin protects the processed tissue. I'm just old school I guess. I don't know if this has been vaildated, I will have to ask our Lead when she gets back from vacation.
Thanks for teaching me my something new today!

Cassie Davis
Histology Department
Anatomical Pathology Laboratory
302-575-8095
Email:  CDavis at che-east.org<mailto:CDavis at che-east.org>

________________________________
From: Perl , Alison <aperl at caremount.com>
Sent: Tuesday, September 3, 2019 12:42 PM
To: Cassie P. Davis <CDavis at che-east.org>
Cc: 'Cassie P. Davis via Histonet' <histonet at lists.utsouthwestern.edu>
Subject: [External] RE: Reprocessing tissue

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Hi Cassie
How often is this happening? We have done it this way, with no ill effects on the processor, but it comes up maybe once every few months - if you have to reprocess tissue daily or weekly, I could see it adding up to a problem. We melt the block, blot the tissue and cassette with gauze to remove as much paraffin as possible, then put in the rack with new work, and usually comes out fine.

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
(914) 302-8424
aperl at caremount.com

-----Original Message-----
From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, September 03, 2019 1:32 PM
To: histonet
Subject: [EXTERNAL] [Histonet] Reprocessing tissue

Has anyone had success with just melting down unfaced, underprocessed blocks for reprocessing and sticking them in NBF for processing without taking them through the clean cycle? The senior tech is doing this and I am concerned about our processor getting paraffin in the NBF and other things.

Cassie Davis

Email:  CDavis at che-east.org<mailto:CDavis at che-east.org>

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