[Histonet] Cell block processing

Fri Oct 25 16:08:11 CDT 2019

I agree with Joe.  We used to use ETOH for cell blocks, but stopped using it when we started doing IHC biomarker testing on these specimens.  Alcohol is good for some proteomic targets, but can be a disaster for others.  We also fix all of our cell block specimens that are collected in saline or RPMI in formalin.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
Richard.cartun at hhchealth.org

-----Original Message-----
From: Joe W. Walker, Jr. via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, October 25, 2019 4:36 PM
To: Charles Riley
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Cell block processing

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As a cytotech, that wouldn’t be my first choice for collections and FNA specimens.  The main reason is that once fixed in 95% ETOH you are limited if you need to perform IHC stains on the cell block unless you have validated your IHCs on ETOH fixed specimens.  How do you process the FNA rinses that in in ETOH: Only Cell blocks or do you have another cytology liquid prep?

Without knowing your prep process, I’d suggest collecting the FNA needle rinses in Hank’s Balanced Salt solution.  After making the cell block, you could then formalin fix them.  I can send you a procedure that we utilize for this process.  The cell blocks cut great, look great, and you can perform IHC an molecular testing if needed.

Joe Walker

From: Charles Riley <criley at dpspa.com>

Sent: Friday, October 25, 2019 12:57 PM

To: Joe W. Walker, Jr. <jwwalker at rrmc.org>

Cc: histonet at lists.utsouthwestern.edu

Subject: Re: [Histonet] Cell block processing

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Our tech said they use 95% alcohol to collect the specimen.

On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr. <jwwalker at rrmc.org<mailto:jwwalker at rrmc.org>> wrote:

Hi Charles,

What are you collecting the FNA into?  Cytorich? Cytolyt? Other?

Joe W. Walker, Jr. MS, SCT(ASCP)

Anatomical Pathology Manager

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-----Original Message-----

From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>

Sent: Friday, October 25, 2019 8:13 AM

To: Histo List <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>

Subject: [Histonet] Cell block processing

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Does anyone have any tips or suggestions on how to better process extremely

bloody FNA  specimens?    Is there anyway to clear out some or all of the

blood without destroying the other tissues?

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs

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--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs

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